mProX™ Human PTK2B Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-1222-KX463	Magic™ Human PYK2(PTK2B) in Vitro Assay	Human		Kinase Assay

Product Information

Target Family

Kinases/Enzyme

Target Protein Species

Human

Host Cell Type

HEK293;CHO-K1;T cells

Target Classification

Kinase Cell Lines

Target Research Area

Cancer Research

Related Diseases

Osteoporosis; Osteopetrosis

Gene ID

Human:2185

UniProt ID

Human:Q14289

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

PTK2B, also known as protein tyrosine kinase 2 beta, is a gene that has been studied in various contexts. In the context of low bone mineral density in postmenopausal women, PTK2B was found to be upregulated in individuals with osteopenia and osteoporosis compared to those with normal bone mineral density. It was identified as a potential candidate biomarker for the early diagnosis of osteoporosis. In the context of polycystic ovary syndrome (PCOS), PTK2B was found to be upregulated in granulosa cells from patients with PCOS compared to controls. It was implicated in the pathogenesis of PCOS and was associated with NLRP3 inflammasome-dependent pathway activation. In the context of lung adenocarcinoma, PTK2B was identified as a hub gene in a prognostic signature. High expression of PTK2B was associated with reduced survival rates and lower levels of immune cell infiltration. In the context of Hashimoto's thyroiditis, PTK2B was identified as a hub gene associated with immune and oxidative stress dysregulation. Its expression was positively correlated with serum antibody levels and inflammatory infiltration scores. Finally, in the context of gastrointestinal nematode infection in sheep, PTK2B was identified as a candidate gene associated with the biological response to infection. These studies highlight the diverse applications of PTK2B in different disease contexts.

Protocols

Please visit our protocols page.

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FAQ

chat Peyton Brown (Verified Customer)

How does PTK2B affect hepatocellular carcinoma cell proliferation? Dec 09 2022

chat Patrick Liam (Creative Biolabs Scientific Support)

MicroRNA214 inhibits HCC cell proliferation through targeting PTK2b/Pyk2, indicating its role in liver cancer cell proliferation. Dec 09 2022

chat Alex Davis (Verified Customer)

What is the role of PTK2B in macrophage homeostasis during tuberculosis infection? Sep 29 2023

chat Patrick Liam (Creative Biolabs Scientific Support)

SIRPα maintains macrophage homeostasis by interacting with PTK2B kinase in Mycobacterium tuberculosis infection. Sep 29 2023

Published Data

Fig.1 Knockdown of PTK2B in CD4+ T cells.

The depletion of c-SRC and PTK2B proteins was achieved through electroporation of 1.0 x 10^7 enriched CD4+ T cells with 900 nmol/l of siRNA, either non-targeting (NT), c-SRC-targeting, or PTK2B-targeting, 48 hours post-electroporation.

Ref: McCarthy, Stephen DS, et al. "c-SRC protein tyrosine kinase regulates early HIV-1 infection post-entry." Aids 30.6 (2016): 849-858.

Pubmed: 26807966

DOI: 10.1097/QAD.0000000000001028

Research Highlights

Wang, Bo. et al. "NLRP3 Inflammasome-dependent Pathway is Involved in the Pathogenesis of Polycystic Ovary Syndrome." Reproductive sciences (Thousand Oaks, Calif.), 2023.
Research has demonstrated the importance of inflammation in the development of polycystic ovary syndrome (PCOS). The NLRP3 inflammasome, a protein complex, has been found to play a crucial role in inflammation. This study aimed to examine the expression and mechanisms of the NLRP3 inflammasome in PCOS. Human granulosa cells (GCs) were collected from PCOS patients and control women who underwent in vitro fertilization. Additionally, ovarian tissue was obtained from mice with PCOS-like changes. RNA sequencing and PCR were used to analyze gene expression. The results showed that NLRP3 and caspase-1 expression were significantly higher in GCs from PCOS patients compared to the control group. Similar increases were observed in a mouse model of PCOS. There was also an elevated level of the inflammatory molecule IL-18 in mice with PCOS-like changes. Overexpression of NLRP3 in a GC line resulted in the upregulation of genes related to N-glycan processing, steroidogenesis, oocyte maturation, autophagy, and apoptosis. These findings suggest that the NLRP3 inflammasome is involved in the development of PCOS by promoting pyroptosis and influencing key processes in GCs.
Wang, Bo. et al. "NLRP3 Inflammasome-dependent Pathway is Involved in the Pathogenesis of Polycystic Ovary Syndrome." Reproductive sciences (Thousand Oaks, Calif.), 2023.
Pubmed: 37815748 DOI: 10.1007/s43032-023-01348-z

Zhang, Wentao. et al. "Identification of cuproptosis and immune-related gene prognostic signature in lung adenocarcinoma." Frontiers in immunology, 2023.
Cuproptosis has recently emerged as a distinct form of programmed cell death, distinct from pyroptosis, ferroptosis, and autophagy. It has shown great potential as a target for cancer therapy. Furthermore, the role of immune-related genes in cancer progression and patient prognosis cannot be ignored. To this end, this study aimed to develop a survival prediction model for lung adenocarcinoma patients by considering cuproptosis and immune-related genes. The utilization of this model could improve the efficacy of personalized treatment for these patients.
Zhang, Wentao. et al. "Identification of cuproptosis and immune-related gene prognostic signature in lung adenocarcinoma." Frontiers in immunology, 2023.
Pubmed: 37622116 DOI: 10.3389/fimmu.2023.1179742