mProX™ Human PRKG2 Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-1122-KX1078	Magic™ Human CGK2(PRKG2) in Vitro Assay	Human		Kinase Assay

Product Information

Target Protein

PRKG2

Target Family

Kinases/Enzyme Drug Discovery Assays and Products

Target Protein Species

Human

Host Cell Type

CHO-K1; HEK293

Target Classification

Kinase Cell Lines

Related Diseases

Spondylometaphyseal Dysplasia, Pagnamenta Type and Acromesomelic Dysplasia 4. Among its related pathways are RAF/MAP kinase cascade and Metabolism of water-soluble vitamins and cofactors

Gene ID

5593

UniProt ID

Q13237

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The prkg2 gene encodes the cGMP-dependent protein kinase 2, a member of the serine/threonine kinase family. The regulation of endochondral ossification and the proliferation of gastrointestinal and nervous system cells are crucial functions of the prkg2 gene, which is extensively expressed in the brain, intestinal mucosa, and lungs. Prkg2 is extensively expressed in lung epithelial cells, particularly in alveolar type 2 (AT2) cells, according to earlier research. Differentiated AT2 cells have also shown a high expression level of prkg2 throughout lung development. Prkg2 has been demonstrated to stop lung cancer cells from growing, but it is unclear how prkg2 affects alveolar epithelial cells' homeostasis. The customized PRKG2 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Sandra

High quality and reliable PRKG2 cell line. Apr 20 2021

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chat Dorothy

This PRKG2 cell line saved us time and resources. Plus, the technical support from the company was outstanding-prompt and helpful. May 26 2023

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FAQ

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Published Data

Fig.1 Expression and function of type II cGMP-dependent protein kinase (Prkg2) in the oil-stimulated mouse uterus.

Prkg2 mRNA and protein levels in the decidualizing stromal tissues under oil-infused artificial decidualization were markedly elevated. Meanwhile, following artificially induced decidualization, immunohistochemistry data demonstrated that Prkg2 expression was greatly elevated in the stroma.

Ref: Zhang, Yang, et al. "cGMP-dependent protein kinase II determines β-catenin accumulation that is essential for uterine decidualization in mice." American Journal of Physiology-Cell Physiology 317.6 (2019): C1115-C1127.

Pubmed: 31509448

DOI: 10.1152/ajpcell.00208.2019

Research Highlights

According to this study, achondroplastic dwarfism-related enhanced proliferation and apoptosis in the growth plate are caused by PRKG2 function loss, which also causes differential expression of P53-regulated genes and other pathways.
Koltes, James E., et al. "Transcriptional profiling of PRKG2-null growth plate identifies putative down-stream targets of PRKG2." BMC research notes 8.1 (2015): 1-10.
Pubmed: 25924610 DOI: 10.1186/s13104-015-1136-6

These results imply that demyelination is related to PRKG2-regulated phosphorylation of MYRF, which may be a target for therapy to lessen demyelination.
Yin, Peng, et al. "Phosphorylation of myelin regulatory factor by PRKG 2 mediates demyelination in Huntington's disease." EMBO reports 21.6 (2020): e49783.
Pubmed: 32270922 DOI: 10.15252/embr.201949783