mProX™ Human PRKCG Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-1222-KX443	Magic™ Human PKCγ(PRKCG) in Vitro Assay	Human		Kinase Assay

Product Information

Target Family

Kinases/Enzyme

Target Protein Species

Human

Host Cell Type

HEK293;CHO-K1

Target Classification

Kinase Cell Lines

Target Research Area

Metabolic Research

Related Diseases

Spinocerebellar Ataxia 14; Hereditary Ataxia

Gene ID

Human:5582

UniProt ID

Human:P05129

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

PRKCG, which encodes Protein Kinase C gamma (PKCŒ≥), has been implicated in various biological processes and diseases. In a study on late-onset spinocerebellar atrophy type 14, mutations in PKCŒ≥ were found to be the cause of the disease. Another study investigated the role of PKCŒ≥ in aflatoxin B1 (AFB1)-mediated toxicity in porcine alveolar macrophages (PAM). The study revealed that AFB1 exposure led to the activation of the calcium signaling pathway through the ceRNA regulatory network, involving the competitive binding of specific microRNAs and the upregulation of PKCŒ≥. In the context of postoperative pain, the interaction between NETO2 and GluK2 was found to contribute to pain hypersensitivity by inducing PKCŒ≥ activation and the synaptic incorporation of AMPA receptor GluR1 subunits in the rat dorsal horn. Additionally, PKCŒ≥ fusion genes were identified in melanocytic neoplasms, suggesting a role for PKCŒ≥ in the pathogenesis of these tumors. Finally, a viewpoint article discussed the involvement of PKCŒ≥ in spinocerebellar ataxias, proposing that dysfunction of Purkinje cells, rather than their loss, may be the primary driver of these diseases. Overall, these studies highlight the diverse applications of PRKCG in various biological processes and diseases.

Protocols

Please visit our protocols page.

Customer Reviews

There are currently no Customer reviews or questions for mProX™ Human PRKCG Stable Cell Line (S01YF-1023-PY63). Click the button above to contact us or submit your feedback about this product.

FAQ

chat Casey Davis (Verified Customer)

What is the role of PRKCG in ataxia? Mar 29 2023

chat Patrick Liam (Creative Biolabs Scientific Support)

Novel variants in PRKCG have been linked to the pathogenesis of spinocerebellar ataxia type 14. Mar 29 2023

chat Jordan Jones (Verified Customer)

How does PRKCG mutation affect Parkinson's disease? Feb 15 2023

chat Patrick Liam (Creative Biolabs Scientific Support)

A de novo PRKCG mutation has been associated with early-onset Parkinson's disease with atypical molecular imaging abnormalities. Feb 15 2023

Published Data

Fig.1 The modulation of the autophagic rate is not achievable by the PRKCG isoform.

LC3 cleavage and lipidation were quantified through immunoblot analysis using an LC3-specific antibody in cells expressing PRKCG-HA (PRKCG), PRKCA (PRKCA), PRKCB (PRKCB), or mock (CTRL). The specific protein levels were examined in the cell lysates, and the presence of PRKCG-HA was identified via Western blot analysis for HA, with ACTB serving as the loading control. Band intensity was measured, and the LC3-II/LC3-I and LC3-II/ACTB ratios were determined.

Ref: Patergnani, Simone, et al. "PRKCB/protein kinase C, beta and the mitochondrial axis as key regulators of autophagy." Autophagy 9.9 (2013): 1367-1385.

Pubmed: 23778835

DOI: 10.4161/auto.25239

Research Highlights

Ogawa, Kyoka. et al. "An autopsy case of late-onset spinocerebellar atrophy type 14." Neuropathology and applied neurobiology, 2023.
This article is a short communication about an autopsy case of a rare neurological disorder called spinocerebellar atrophy type 14 (SCA14). The authors report the clinical and pathological features of a 67-year-old Japanese woman who had progressive cerebellar ataxia and cognitive impairment. They found that she had a mutation in the PRKCG gene, which is associated with SCA14, and that her brain showed severe degeneration of the cerebellum and other regions. They also discuss the possible mechanisms and implications of their findings.
Ogawa, Kyoka. et al. "An autopsy case of late-onset spinocerebellar atrophy type 14." Neuropathology and applied neurobiology, 2023.
Pubmed: 37705255 DOI: 10.1111/nan.12936

Tian, Yu. et al. "Aflatoxin B1 affects porcine alveolar macrophage growth through the calcium signaling pathway mediated by the ceRNA regulatory network." Molecular biology reports, 2023.
Aflatoxin B1 (AFB1) is a common contaminant found in human and animal food, known to impair the immune system. Despite its prevalence, there is currently a lack of understanding regarding the mechanisms behind AFB1-induced toxicity in macrophages. Further research is needed to elucidate these mechanisms and potentially develop interventions to mitigate the harmful effects of AFB1 on the immune system.
Tian, Yu. et al. "Aflatoxin B1 affects porcine alveolar macrophage growth through the calcium signaling pathway mediated by the ceRNA regulatory network." Molecular biology reports, 2023.
Pubmed: 37572211 DOI: 10.1007/s11033-023-08672-2