mProX™ Human PRKCD Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-1222-KX444	Magic™ Human PKCδ(PRKCD) in Vitro Assay	Human		Kinase Assay

Product Information

Target Family

Kinases/Enzyme

Target Protein Species

Human

Host Cell Type

HEK293;CHO-K1;A2780

Target Classification

Kinase Cell Lines

Target Research Area

Autoimmune Research

Related Diseases

Autoimmune Lymphoproliferative Syndrome, Type Iii; Systemic Lupus Erythematosus 16

Gene ID

Human:5580

UniProt ID

Human:Q05655

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

PRKCD, also known as protein kinase C delta, has various applications in different fields. In the field of cancer research, a study found that TRIM69, a protein, can suppress the resistance to cell death (anoikis) and metastasis in gastric cancer cells by promoting the degradation of PRKCD through a process called ubiquitin-proteasome-mediated degradation. This suggests that targeting PRKCD could be a potential therapeutic approach for metastatic gastric cancer. Additionally, PRKCD has been implicated in the antioxidant and anti-inflammatory properties of lipophilic extracts from Polyscias fruticosa leaves and Liriope platyphylla seeds. These extracts contain compounds that regulate PRKCD and other target genes involved in oxidative stress and inflammation-related pathways. The antioxidant and anti-inflammatory effects of these extracts make them promising candidates for the development of functional foods and nutraceuticals. Furthermore, PRKCD deficiency has been linked to systemic lupus erythematosus and other autoimmune and immunodeficiency disorders. Understanding the role of PRKCD in immune tolerance and effector functions against pathogens is crucial for the diagnosis and management of these conditions. Overall, PRKCD has diverse applications in cancer research, natural product development, and immunology.

Protocols

Please visit our protocols page.

Customer Reviews

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FAQ

chat Cameron Smith (Verified Customer)

How does PRKCD regulate basal mitophagy? Apr 22 2023

chat Patrick Liam (Creative Biolabs Scientific Support)

PRKCD localizes to mitochondria and regulates the recruitment of ULK1-ATG13 during the induction of mitophagy. Apr 22 2023

chat Skyler Smith (Verified Customer)

What is the role of PRKCD in chronic obstructive pulmonary disease? Oct 17 2020

chat Patrick Liam (Creative Biolabs Scientific Support)

PRKCD might be a potential therapeutic target for COPD by promoting inflammatory response, apoptosis, mitochondrial dysfunction, and MUC5AC hypersecretion. Oct 17 2020

Published Data

Fig.1 The proliferation of A2780 cells was assessed using the CCK-8 assay.

Following PRKCD knockdown, a notable inhibition of A2780 proliferation was observed when compared to the control group.

Ref: Yao, Lijuan, et al. "MiR181c inhibits ovarian cancer metastasis and progression by targeting PRKCD expression." International journal of clinical and experimental medicine 8.9 (2015): 15198.

Pubmed: 26629004

DOI: NA

Research Highlights

Sun, Linqing. et al. "TRIM69 suppressed the anoikis resistance and metastasis of gastric cancer through ubiquitin,Äíproteasome-mediated degradation of PRKCD." Oncogene, 2023.
The tripartite motif (TRIM) protein family has been extensively studied in various human cancers, including gastric cancer (GC). However, the specific role of TRIM69 in the resistance to anoikis and metastasis of GC cells has yet to be determined. Through RNA-sequencing analysis, differentially expressed genes were identified in anoikis-resistant GC cells. The interaction between TRIM69 and PRKCD was further investigated through coimmunoprecipitation and mass spectrometry. The results revealed that TRIM69 was significantly downregulated in anoikis-resistant GC cells. Overexpression of TRIM69 effectively suppressed anoikis resistance and metastasis of GC cells in both in vitro and in vivo experiments, while silencing TRIM69 had the opposite effects. Mechanistically, TRIM69 was found to interact with PRKCD via its B-box domain and induce K48-linked polyubiquitination of PRKCD. Furthermore, TRIM69 also inhibited the production of BDNF in a PRKCD-dependent manner. Of note, the overexpression of PRKCD or BDNF counteracted the effects of TRIM69 on anoikis resistance and metastasis of GC cells. Interestingly, a TRIM69 mutant lacking ubiquitin E3 ligase activity failed to inhibit BDNF production.
Sun, Linqing. et al. "TRIM69 suppressed the anoikis resistance and metastasis of gastric cancer through ubiquitin,Äíproteasome-mediated degradation of PRKCD." Oncogene, 2023.
Pubmed: 37864033 DOI: 10.1038/s41388-023-02873-6

H G Rarison, Razanamanana. et al. "Antioxidant and Anti-Inflammatory Mechanisms of Lipophilic Fractions from Polyscias fruticosa Leaves Based on Network Pharmacology, In Silico, and In Vitro Approaches." Foods (Basel, Switzerland), 2023.
This research paper examines the relationship between social media usage and academic performance among college students. Data was gathered through surveys and interviews, revealing a negative impact of social media on students' academic performance. The study suggests that promoting awareness among students about managing their social media usage and implementing limits can help mitigate the distracting effects on academic responsibilities.
H G Rarison, Razanamanana. et al. "Antioxidant and Anti-Inflammatory Mechanisms of Lipophilic Fractions from Polyscias fruticosa Leaves Based on Network Pharmacology, In Silico, and In Vitro Approaches." Foods (Basel, Switzerland), 2023.
Pubmed: 37835296 DOI: 10.3390/foods12193643