mProX™ Human PRKACG Stable Cell Line
- Product Category:
- Membrane Protein Stable Cell Lines
- Subcategory:
- Kinase Cell Lines
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Published Data
Fig.1 Alterations in the subcellular localization of BACE1 and EEA1 were observed in HeLa cells following knockdown of the kinase.
HeLa cells that had been grown on glass-based dishes were transfected with 50 nm siRNA against a control protein (eGFP), PRKACG, or GSK3β. The cells were further transfected with 1 µg/mL BACE1-myc plasmid at 48 h after transfection with the siRNAs. After further incubation for 24 h, the cells were fixed and dual-immunostained with anti-myc and anti-EEA1 antibodies, followed by Alexa 488- or Cy3-conjugated secondary antibodies, respectively. The samples were viewed using an LSM510 confocal microscope with a 63x Plan-Neofluar oil immersion objective. Scale bar = 10 µm.
Ref: Adachi, Atsuhiro, et al. "Visual screening and analysis for kinase-regulated membrane trafficking pathways that are involved in extensive β-amyloid secretion." Genes to Cells 14.3 (2009): 355-369.
Pubmed: 19210549
DOI: 10.1111/j.1365-2443.2008.01274.x
Research Highlights
D Dergunov, Alexander. et al. "Differential Expression of Subsets of Genes Related to HDL Metabolism and Atherogenesis in the Peripheral Blood in Coronary Artery Disease." Current issues in molecular biology, 2023.
In this study, researchers investigated the differential expression of genes (DEGs) in 76 male patients with coronary artery disease (CAD) and 63 control patients. They also explored the correlation between transcript level and high-density lipoprotein cholesterol (HDL-C). The transcript level of 24 genes related to HDL metabolism and 41 genes related to atherosclerosis-prone was measured using real-time RT-PCR on RNA extracted from peripheral blood mononuclear cells. A total of 28 DEGs were discovered, with upregulation of cholesterol transporters, lipoprotein metabolism genes, and inflammation-regulating genes observed in CAD patients.
D Dergunov, Alexander. et al. "Differential Expression of Subsets of Genes Related to HDL Metabolism and Atherogenesis in the Peripheral Blood in Coronary Artery Disease." Current issues in molecular biology, 2023.
Pubmed:
37623250
DOI:
10.3390/cimb45080431
Ye, Xian-Wen. et al. "Exploring the common pathogenesis of Alzheimer's disease and type 2 diabetes mellitus via microarray data analysis" Frontiers in aging neuroscience, 2023.
The incidence of Alzheimer's Disease (AD) and Type 2 Diabetes Mellitus (DM) is on the rise in contemporary society. However, despite mounting evidence linking DM to an increased risk of AD, the exact mechanisms underlying this relationship have yet to be fully understood. Further research is needed to elucidate the interrelationships between these two diseases.
Ye, Xian-Wen. et al. "Exploring the common pathogenesis of Alzheimer's disease and type 2 diabetes mellitus via microarray data analysis" Frontiers in aging neuroscience, 2023.
Pubmed:
36923118
DOI:
10.3389/fnagi.2023.1071391
