mProX™ Human PRKACB Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-1222-KX438	Magic™ Human PKACβ(PRKACB) in Vitro Assay	Human		Kinase Assay

Product Information

Target Family

Kinases/Enzyme

Target Protein Species

Human

Host Cell Type

HEK293;CHO-K1

Target Classification

Kinase Cell Lines

Target Research Area

Cardiovascular Research

Related Diseases

Cardioacrofacial Dysplasia 2; Ellis-Van Creveld Syndrome

Gene ID

Human:5567

UniProt ID

Human:P22694

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

PRKACB (protein kinase cAMP-activated catalytic subunit beta) has several applications. In the context of pancreatobiliary oncocytic neoplasms, gene rearrangement and expression of PRKACB play a role in the morpho-biology of these tumors. PRKACB fusion genes are characteristic drivers of intraductal oncocytic papillary neoplasms (IOPNs) and are associated with upregulation of PRKACB and PRKACA, as well as phosphorylated-CREB. PRKACB also has implications in coronary artery disease, where its expression is upregulated in peripheral blood mononuclear cells of patients with CAD. In the context of oligoasthenozoospermia, PRKACB is upregulated in response to treatment with hyperoside, leading to improvements in sperm density and testicular function. Additionally, PRKACB is involved in the mechanism of Moringa oleifera leaf extract in treating constipation, modulating signaling pathways related to gastrointestinal motility and intestinal fluid secretion. Finally, PRKACB is implicated in augmented renal clearance, where its expression is altered along with other genes and metabolites associated with ARC. Overall, PRKACB is involved in various biological processes and may have potential therapeutic implications in different conditions.

Protocols

Please visit our protocols page.

Customer Reviews

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FAQ

chat Morgan Garcia (Verified Customer)

What is the significance of PRKACB in oncocytic tumors of the pancreatobiliary system? Mar 17 2021

chat Patrick Liam (Creative Biolabs Scientific Support)

PRKACB fusion genes play a role in the development of intraductal oncocytic papillary neoplasms of the pancreas and bile duct. Mar 17 2021

chat Peyton Williams (Verified Customer)

How does PRKACB affect tau phosphorylation in nerve cells? Jan 12 2022

chat Patrick Liam (Creative Biolabs Scientific Support)

miR-200a-3p regulates PRKACB in the PKA/CREB pathway, leading to abnormal phosphorylation of tau protein in nerve cells. Jan 12 2022

Published Data

Fig.1 The results of the IPA analysis were obtained for the 69 genes that were shared between the cells subjected to AK1 silencing and those subjected to PRKACB silencing.

The underlying mechanism responsible for the positive regulation of HIV-1 LTR activity by AK1 and PRKACB was investigated through the application of DNA microarray analysis to HEK293T cells in which AK1 or PRKACB had been silenced. A total of 132 genes and 93 genes exhibited significant alterations in expression levels (with a difference greater than 50% and P < 0.05) following AK1 and PRKACB knockdown, respectively. A set of 69 genes, overlapping between AK1 and PRKACB silenced HEK293T cells, was subjected to Ingenuity Pathway Analysis (IPA). The IPA results revealed that several amino acid biosynthesis pathways, the TGF-β signaling pathway, and the p53 signaling pathway were found to be significantly altered subsequent to AK1 and PRKACB knockdown.

Ref: Jiang, Wei-Min, et al. "A high throughput RNAi screen reveals determinants of HIV-1 activity in host kinases." International journal of clinical and experimental pathology 7.5 (2014): 2229.

Pubmed: 24966931

DOI: NA

Research Highlights

D Dergunov, Alexander. et al. "Differential Expression of Subsets of Genes Related to HDL Metabolism and Atherogenesis in the Peripheral Blood in Coronary Artery Disease." Current issues in molecular biology, 2023.
The study aimed to investigate the differential expression of genes (DEGs) in coronary artery disease (CAD), as well as the link between transcript level and high-density lipoprotein cholesterol (HDL-C). A total of 76 male patients with CAD and 63 control patients were included. The transcript levels of 24 genes related to HDL metabolism and 41 genes related to atherosclerosis-prone were measured in RNA isolated from peripheral blood mononuclear cells using real-time RT-PCR. A total of 28 DEGs were identified, including significant downregulation of cholesterol transporters ABCC6 and ABCA1 in CAD patients.
D Dergunov, Alexander. et al. "Differential Expression of Subsets of Genes Related to HDL Metabolism and Atherogenesis in the Peripheral Blood in Coronary Artery Disease." Current issues in molecular biology, 2023.
Pubmed: 37623250 DOI: 10.3390/cimb45080431

Fan, Qigang. et al. "Studying the effect of hyperoside on recovery from cyclophosphamide induced oligoasthenozoospermia." Systems biology in reproductive medicine, 2023.
Oligoasthenozoospermia is a growing concern with limited treatment options. One potential solution is the use of hyperoside, a compound found in traditional Chinese medicine. In this study, researchers utilized a mouse model to investigate the effects of hyperoside on oligoasthenozoospermia induced by cyclophosphamide. The mice were divided into four groups: a control group, a treatment control group, a disease group, and a treatment group. The mice were evaluated for body and testicular weight, sperm parameters, and testicular histology. The results showed that treatment with hyperoside significantly improved sperm density, viability, and testicular function. Additionally, hyperoside was found to increase testosterone production and regulate the expression of various genes involved in spermatogenesis. Overall, it was concluded that hyperoside has potential as a protective treatment for oligoasthenozoospermia by regulating testosterone production and promoting healthy sperm maturation.
Fan, Qigang. et al. "Studying the effect of hyperoside on recovery from cyclophosphamide induced oligoasthenozoospermia." Systems biology in reproductive medicine, 2023.
Pubmed: 37578152 DOI: 10.1080/19396368.2023.2241600