mProX™ Human PPARD Stable Cell Line

Product Information

Target Protein

PPARD

Target Family

Peroxisome Proliferator-activated Receptor

Target Protein Species

Human

Host Cell Type

HepG2; CHO-K1; HEK293

Target Classification

Nuclear Receptor

Target Research Area

Diabetes Research; Metabolic Research

Related Diseases

Diabetic Cataract; Abdominal Obesity-Metabolic Syndrome

Gene ID

5467

UniProt ID

Q03181

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

Nuclear receptors such as peroxisome proliferator-activated receptor delta (PPAR-delta) are encoded by the PPARD gene in humans. Numerous biological processes are regulated by PPAR-delta, which may also have a role in the emergence of multiple chronic illnesses, such as diabetes, obesity, atherosclerosis, and cancer. Exercise increases muscle PPARD expression, which raises oxidative capacity (the ability to burn fat) and increases type I fibers. Agonists for AMPK and PPAR-delta are considered to be exercise mimics. PPAR-β/δ raises oxidation and uncouples oxidative phosphorylation in adipose tissue. Studies using animals lacking this protein have indicated that it is involved in the myelination of the corpus callosum, the proliferation of epidermal cells, and the metabolism of fats and carbohydrates. The customized PPARD stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Jessica

I think this PPARD cell line is better than many similar products already on the market. Apr 08 2023

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chat Michael

This is a very reliable PPARD KO cell line with high sensitivity and specificity, suitable for experiments. Apr 17 2023

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FAQ

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Published Data

Fig.1 Induction of the expression of PPAR by doxycycline in established cell lines.

For five days, these cell lines were grown in the presence of Dox at concentrations of 0, 0.01, 0.1, or 1000 ng/ml, which promoted the expression of PPARs in a dose-dependent way.

Ref: Tachibana, Keisuke, et al. "Gene expression profiling of potential peroxisome proliferator-activated receptor (PPAR) target genes in human hepatoblastoma cell lines inducibly expressing different PPAR isoforms." Nuclear receptor 3 (2005): 1-17.

Pubmed: 16197558

DOI: 10.1186/1478-1336-3-3

Research Highlights

Although the expression of peroxisome proliferator-activated receptor-δ (PPARD) is elevated in several major human malignancies, its involvement in metastasis in cancer cells is still unclear.
Zuo, Xiangsheng, et al. "Metastasis regulation by PPARD expression in cancer cells." JCI insight 2.1 (2017).
Pubmed: 28097239 DOI: 10.1172/jci.insight.91419

APC mutations trigger abnormal β-catenin signaling, which initiates colorectal cancer; however, extra molecular processes are needed for colorectal cancer growth. A downstream target of β-catenin, PPAR-delta (PPARD), is increased in colorectal cancer.
Liu, Yi, et al. "Pleiotropic effects of PPARD accelerate colorectal tumorigenesis, progression, and invasion." Cancer research 79.5 (2019): 954-969.
Pubmed: 30679176 DOI: 10.1158/0008-5472.CAN-18-1790