mProX™ Human PNCK Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-1122-KX1048	Magic™ Human CaMK1β(PNCK) in Vitro Assay	Human		Kinase Assay

Product Information

Target Protein

PNCK

Target Family

Kinases/Enzyme Drug Discovery Assays and Products

Target Protein Species

Human

Host Cell Type

RCC; CHO-K1; HEK293

Target Classification

Kinase Cell Lines

Target Research Area

Reproductive Research

Related Diseases

Syndromic X-Linked Intellectual Disability Lubs Type. Among its related pathways are B Cell Receptor Signaling Pathway and Development EGFR signaling pathway

Gene ID

139728

UniProt ID

Q6P2M8

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

A protein-coding gene is called PNCK (Pregnancy Up-Regulated Nonubiquitous CaM Kinase). PNCK-related illnesses include Lubs Type Syndromic X-Linked Intellectual Disability. The development EGFR signaling route and the B cell receptor signaling pathway are two of its associated pathways. This gene's Gene Ontology (GO) annotations include protein tyrosine kinase activity, transferase activity, and transferring phosphorus-containing groups. A putative calcium-triggered signaling cascade protein kinase that is dependent on calcium and calcium modulin. phosphorylates SYN1/synapsin I and CREB1 in vitro. It also phosphorylates and activates CAMK1. The customized PNCK stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Laura

I've been using the PNCK cell line for my studies, and it has been an excellent choice. Feb 27 2022

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chat Jason

The human PNCK overexpression cell line showed good performance in our hands. It was reliable for most experiments, and the customer support team was helpful in troubleshooting. Oct 27 2021

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FAQ

Any questions about our products? Please visit our frequently asked questions page.

Published Data

Fig.1 Characterization of PNCK overexpressing RCC cell lines.

According to STAR techniques, PNCK was consistently overexpressed in 786-O, A498, ACHN, and Caki-1. Immunofluorescence, western blot, and qPCR were used to confirm PNCK overexpression. Using immunofluorescence, changes in phospho-CREB expression were examined.

Ref: Essegian, Derek J., et al. "Cellular and molecular effects of PNCK, a non-canonical kinase target in renal cell carcinoma." Iscience 25.12 (2022).

Pubmed: 36465101

DOI: 10.1016/j.isci.2022.105621

Research Highlights

This work shows, for the first time, that PNCK knockdown can inhibit NPC cell growth and trigger apoptosis in both vitro and in vivo via controlling the PI3K/AKT/mTOR signaling pathway. These results imply that PNCK may provide a unique therapeutic target for the management of NPC.
Xu, Yuanji, et al. "PNCK depletion inhibits proliferation and induces apoptosis of human nasopharyngeal carcinoma cells in vitro and in vivo." Journal of Cancer 10.27 (2019): 6925.
Pubmed: 31839828 DOI: 10.7150/jca.33698

In HCC, it was shown that PNCK expression was upregulated and that this was associated with a poor prognosis. PNCK may be a viable candidate target for therapy as well as a predictive biomarker for HCC.
Cho, Yoon Ah, et al. "Expression of pregnancy up-regulated non-ubiquitous calmodulin kinase (PNCK) in hepatocellular carcinoma." Cancer Genomics & Proteomics 17.6 (2020): 747-755.
Pubmed: 33099476 DOI: 10.21873/cgp.20229