mProX™ Human PKN2 Stable Cell Line
- Product Category:
- Membrane Protein Stable Cell Lines
- Subcategory:
- Kinase Cell Lines
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Patrick Liam (Creative Biolabs Scientific Support)
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Published Data
Fig.1 Cell proliferation inhibition is achieved through the downregulation of SNTB1, with dependence on PKN2.
Si-SNTB1, si-PKN2, or a combination of si-SNTB1 and si-PKN2, along with their respective control siRNA (si-Ctrl), were transfected into HCT116 cells. Cell survival was assessed through the execution of a colony formation assay, wherein images were captured, and colony quantification was conducted, subsequently being normalized concerning the survival rate of control cells. Significance was observed at *P < 0.05 and #P < 0.05 concerning si-PKN2.
Ref: Liu, Liya, et al. "Upregulation of SNTB1 correlates with poor prognosis and promotes cell growth by negative regulating PKN2 in colorectal cancer." Cancer Cell International 21 (2021): 1-14.
Pubmed: 34663329
DOI: 10.1186/s12935-021-02246-7
Research Highlights
Ribeiro-Dos-Santos, Arthur. et al. "The fusiform gyrus exhibits differential gene-gene co-expression in Alzheimer's disease." Frontiers in aging neuroscience, 2023.
Alzheimer's Disease (AD) is a well-known irreversible neurodegenerative disease, characterized by the presence of specific protein plaques in the brain. However, the exact causes of AD are still unknown. The fusiform gyrus, a region of the brain, has been linked to mild cognitive impairment, a potential risk factor for AD. In this study, gene co-expression and differential co-expression networks were analyzed in post-mortem fusiform gyrus tissue from healthy and AD individuals using RNA sequencing data. Using large cohorts such as ROSMAP, MSBB, and Mayo, the researchers identified specific gene hubs and pathways associated with AD. Their analysis found 243 genes that make up the differential co-expression networks in AD.
Ribeiro-Dos-Santos, Arthur. et al. "The fusiform gyrus exhibits differential gene-gene co-expression in Alzheimer's disease." Frontiers in aging neuroscience, 2023.
Pubmed:
37255536
DOI:
10.3389/fnagi.2023.1138336
A Zorina, A. et al. "SpkH (Sll0005) from Synechocystis sp. PCC 6803 is an active Mn2+-dependent Ser kinase." Biochimie, 2023.
In the genome of Synechocystis sp. PCC 6803, twelve genes encoding potential serine-threonine protein kinases (STPKs) have been identified and classified into two distinct clusters: the serine/threonine-protein N2-like kinases (PKN2-type) and the "activity of bc1 complex" kinases (ABC1-type), with the former demonstrating known activity while the latter has remained unreported until now. This research focuses on SpkH (Sll0005), initially annotated as an ABC1-type STPK, and reveals its genuine serine protein kinase activity, particularly its preference for casein substrate, with Mn2+ being the most potent activator. Notably, SpkH's activity is effectively inhibited by heparin and spermine but not by staurosporine. The study further identifies a consensus phosphorylation motif, X1X2pSX3E, recognized by SpkH, indicating its resemblance to casein kinases in terms of substrate specificity and responsiveness to certain regulators, marking a pioneering report on the active nature of SpkH in Synechocystis.
A Zorina, A. et al. "SpkH (Sll0005) from Synechocystis sp. PCC 6803 is an active Mn2+-dependent Ser kinase." Biochimie, 2023.
Pubmed:
37209809
DOI:
10.1016/j.biochi.2023.05.006
