mProX™ Human PIM2 Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-1222-KX435	Magic™ Human PIM2 in Vitro Assay	Human		Kinase Assay

Product Information

Target Family

Kinases/Enzyme

Target Protein Species

Human

Host Cell Type

HEK293;CHO-K1;HepG2;Huh-7

Target Classification

Kinase Cell Lines

Target Research Area

Cancer Research

Related Diseases

Colorectal Adenocarcinoma; Lymphoma

Gene ID

Human:11040

UniProt ID

Human:Q9P1W9

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

PIM2, a family of serine/threonine kinases, has been found to play important roles in various diseases and conditions. In the context of cardiovascular disease, PIM kinases have been associated with cell survival, proliferation, and migration. They have widespread expression in cardiovascular tissues and have been implicated in inflammation, thrombosis, and cardiac injury. Inhibition of PIM kinases may be a potential therapeutic approach for treating cardiovascular disease. In the field of pediatric medicine, PIM2 has been used as a predictive tool for assessing the mortality risk in children with severe sepsis and septic shock. It has also been studied in the context of neurocognitive and psychological disorders in children after intensive care admission. Additionally, PIM2 has been investigated in the management of critically ill children with respiratory failure and in the safety and efficacy of continuous renal replacement therapy in children weighing less than 10 kg. Overall, PIM2 has shown promise as a potential target for therapeutic interventions in various disease settings.

Protocols

Please visit our protocols page.

Customer Reviews

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FAQ

chat Cameron Williams (Verified Customer)

How does PIM2 affect glycolysis and cell proliferation in endometriosis? Apr 18 2023

chat Patrick Liam (Creative Biolabs Scientific Support)

PIM2 mediates phosphorylation of PFKFB4, regulating glycolysis and promoting cell proliferation in endometriosis, suggesting its potential as a target for non-contraceptive treatments. Apr 18 2023

chat Jordan Johnson (Verified Customer)

What is the role of PIM2 in multiple myeloma? Dec 31 2020

chat Patrick Liam (Creative Biolabs Scientific Support)

PIM2 kinase plays a pivotal role in plasmablast generation and plasma cell survival in multiple myeloma, making it a potential therapeutic target. Dec 31 2020

Published Data

Fig.1 The impact of proliferation in HepG2 and Huh-7 liver cancer cell lines was assessed through the implementation of PIM2 knockdown.

A profound reduction in cell proliferation was observed in HepG2 cells when subjected to PIM2-specific siRNAs, as revealed by WST-1 assays. Notably, no discernible impact on the PIM2 level was detected with non-specific siRNA transfection (comparison between siCtrl and untreated conditions). Cell proliferation arrest was primarily induced by PIM2 knockdown (upper graph). In contrast, the cessation of Huh-7 cell proliferation was solely evident with the more potent siRNA, siPIM2A, whereas the less potent siRNA, siPIM2B, failed to surpass siCtrl in inhibiting cell proliferation (lower graph).

Ref: Kronschnabl, Pia, et al. "Inhibition of PIM2 in liver cancer decreases tumor cell proliferation in vitro and in vivo primarily through the modulation of cell cycle progression." International journal of oncology 56.2 (2020): 448-459.

Pubmed: 31894300

DOI: 10.3892/ijo.2019.4936

Research Highlights

Nock, Sophie. et al. "Pim Kinases: Important Regulators of Cardiovascular Disease." International journal of molecular sciences, 2023.
The Pim kinase family, consisting of Pim-1, Pim-2, and Pim-3, are constitutively active serine/threonine kinases known for their involvement in cell survival, proliferation, and migration. While previously considered to have overlapping functions, recent research has revealed distinct roles for each isoform. These kinases have primarily been studied in the context of cancer, but there is growing evidence for their involvement in cardiovascular disease, specifically in processes such as inflammation, thrombosis, and cardiac injury. With widespread expression in cardiovascular tissues and upregulation in various cardiovascular risk factors, targeting Pim kinases may be a potential strategy for preventing and treating cardiovascular events in high-risk individuals. This review delves into the expression and activity of Pim kinases in cardiovascular cells, identifies areas for further investigation, and evaluates their therapeutic potential for cardiovascular disease.
Nock, Sophie. et al. "Pim Kinases: Important Regulators of Cardiovascular Disease." International journal of molecular sciences, 2023.
Pubmed: 37511341 DOI: 10.3390/ijms241411582

González, Gustavo. et al. "Lower socioeconomic conditions are associated with higher rates but similar outcomes in Sepsis in children." Andes pediatrica : revista Chilena de pediatria, 2023.
Sepsis is a significant contributor to pediatric morbidity and mortality, particularly in low-income nations. There is a scarcity of data regarding its prevalence in different regions and its correlation with socioeconomic factors. This information is crucial in understanding the impact of sepsis and developing appropriate interventions.
González, Gustavo. et al. "Lower socioeconomic conditions are associated with higher rates but similar outcomes in Sepsis in children." Andes pediatrica : revista Chilena de pediatria, 2023.
Pubmed: 37358112 DOI: 10.32641/andespediatr.v94i2.4452