mProX™ Human PIK3CB Stable Cell Line

Product Information

Target Family

Kinases/Enzyme

Target Protein Species

Human

Host Cell Type

HEK293;CHO-K1;U251

Target Classification

Kinase Cell Lines

Target Research Area

Immunology Research

Related Diseases

Nk-Cell Enteropathy; Immunodeficiency 14

Gene ID

Human:5291

UniProt ID

Human:P42338

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The PIK3CB gene has applications in breast cancer diagnosis as a potential biomarker to complement existing markers such as CEA and CA15-3. High levels of PIK3CB in serum have been associated with breast cancer, and it achieved a specificity of 90.63% in diagnosing breast cancer. Combining PIK3CB with other biomarkers improved the diagnostic efficiency of breast cancer. Additionally, the PIK3CB gene has been implicated in the treatment of calcified aortic valve disease, where berberine, a natural compound, acts on the PIK3CB target within the AGE-RAGE signaling pathway. In the context of mesenchymal stem cells (MSCs) derived from the endometrium of giant pandas, PIK3CB was identified as a hub gene in the differential gene expressions analysis, indicating its potential role in stem cell pluripotency and signaling pathways.

Protocols

Please visit our protocols page.

Customer Reviews

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FAQ

chat Cameron Miller (Verified Customer)

How does PIK3CB contribute to pancreatic cancer progression? Feb 10 2020

chat Patrick Liam (Creative Biolabs Scientific Support)

PIK3CB has been identified as a key factor in the malignant progression of pancreatic cancer, particularly through the miR-532-3p/PIK3CB axis. Feb 10 2020

chat Jordan Miller (Verified Customer)

What is the significance of PIK3CB in Alzheimer's Disease? Aug 28 2021

chat Patrick Liam (Creative Biolabs Scientific Support)

Downregulation of PIK3CB is associated with Alzheimer's Disease, potentially mediated through apoptosis, axon guidance, and the FoxO signaling pathway. Aug 28 2021

Published Data

Fig.1 Synergistic inhibition of proliferation and colony formation in U251 glioblastoma cells was achieved through PTEN restoration and the knockdown of PIK3CB, with no notable effect observed in relation to PIK3CA.

Visualization of anchorage-dependent colony growth was achieved through staining with crystal violet (left panel), followed by subsequent counting and analysis of the colonies (right panel), with values reported as the mean ± standard deviation, performed in triplicate.

Ref: Chen, Hongbo, et al. "PTEN restoration and PIK3CB knockdown synergistically suppress glioblastoma growth in vitro and in xenografts." Journal of neuro-oncology 104 (2011): 155-167.

Pubmed: 21188471

DOI: 10.1007/s11060-010-0492-2

Research Highlights

Mi, Jintao. et al. "FTO, PIK3CB serve as potential markers to complement CEA and CA15-3 for the diagnosis of breast cancer." Medicine, 2023.
The diagnostic efficacy of two commonly used biomarkers, carcinoembryonic antigen (CEA) and carbohydrate antigen 15-3 (CA15-3), is limited in breast cancer (BC). To address this issue, a study was conducted to evaluate the potential of using fat mass and obesity-associated protein (FTO) and phosphatidylinositol-4,5-biphosphate 3-kinase catalytic subunit Œ≤ (PIK3CB) as complementary biomarkers to CEA and CA15-3 in BC diagnosis. The levels of FTO, PIK3CB, CEA, and CA15-3 were measured in 112 BC patients and 64 healthy controls. Spearman's rank correlation analysis and receiver operating characteristic curve (ROC) analysis were performed to evaluate the correlation and diagnostic value of the biomarkers. The results showed that FTO and PIK3CB were significantly elevated in BC patients, and the combination of the four biomarkers improved the diagnostic efficiency of BC. Specifically, the combined panel had a higher area under the curve (AUC) and sensitivity compared to individual biomarkers, making FTO and PIK3CB potential complementary biomarkers for BC diagnosis.
Mi, Jintao. et al. "FTO, PIK3CB serve as potential markers to complement CEA and CA15-3 for the diagnosis of breast cancer." Medicine, 2023.
Pubmed: 37861518 DOI: 10.1097/MD.0000000000035361

Yin, Shi. et al. "Dominoes with interlocking consequences triggered by zinc: involvement of microelement-stimulated MSC-derived exosomes in senile osteogenesis and osteoclast dialogue." Journal of nanobiotechnology, 2023.
In this study, as societal aging intensifies, senile osteoporosis has become a significant global public health concern. Bone microdamage results from an imbalance between osteoclast activity and osteoblast-lineage cell bone formation. Senescent bone marrow mesenchymal stem cell-derived extracellular vesicles stimulate osteoclast differentiation, causing osteoporosis and implant failure. Researchers developed an orthopedic implant with a nutrient coating targeting exosomes, containing high-zinc levels that inhibit bone resorption and induce reprogramming of senile osteogenesis and osteoclast dialogue via exosome modification. Exosomes regulate the PI3K-PIK3CB pathway via microRNA transport, notably miR-146b-5p. In vivo, the nutrient coating improves cancellous bone remodeling and osseointegration by regulating local BMM differentiation, offering a potential solution for age-related osteoporosis.
Yin, Shi. et al. "Dominoes with interlocking consequences triggered by zinc: involvement of microelement-stimulated MSC-derived exosomes in senile osteogenesis and osteoclast dialogue." Journal of nanobiotechnology, 2023.
Pubmed: 37741978 DOI: 10.1186/s12951-023-02085-w