mProX™ Human NR1H4 Stable Cell Line
- Product Category:
- Membrane Protein Stable Cell Lines
- Subcategory:
- Nuclear Receptor
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Published Data
Fig.1 Generation of NR1H4 KO cell lines using CRISPR/CAS9 technology.
A 96-well plate was seeded with GFP-positive single cells, and the cells were then cultured until colonies could be seen. The expression of NR1H4 was detected by immunoblotting the colonies. It was determined that four KO cell line clones (#1-18, #1-20, #1-22, and #2-13) were NR1H4 KO. For additional analysis, one MOCK cell line clone and three of the KO cell line clones (#1-18, #1-20, and #1-22) were utilized.
Ref: Lee, Yun Jeong, et al. "The role of nuclear receptor subfamily 1 group H member 4 (NR1H4) in colon cancer cell survival through the regulation of c-Myc stability." Molecules and cells 43.5 (2020): 459.
Pubmed: 32299194
DOI: 10.14348/molcells.2020.0041
Research Highlights
According to the research, regulating NR1H4 activity in colon cancer cells may be a viable substitute strategy for treating cancer using MYC-targeting medications.
Lee, Yun Jeong, et al. "The role of nuclear receptor subfamily 1 group H member 4 (NR1H4) in colon cancer cell survival through the regulation of c-Myc stability." Molecules and cells 43.5 (2020): 459.
Pubmed:
32299194
DOI:
10.14348/molcells.2020.0041
The main cilia are microtubule-based cellular organelles that have been preserved throughout evolution. They sense metabolic state and connect the sensory system to cellular signaling pathways. Thus, autophagy, which is likewise controlled by nutrient-sensing transcription factors like PPARA (peroxisome proliferator activated receptor alpha) and NR1H4/FXR (nuclear receptor subfamily 1, group H, member 4) is assumed to be closely associated with ciliogenesis.
Liu, Zhi-qiang, et al. "Ciliogenesis is reciprocally regulated by PPARA and NR1H4/FXR through controlling autophagy in vitro and in vivo." Autophagy 14.6 (2018): 1011-1027.
Pubmed:
29771182
DOI:
10.1080/15548627.2018.1448326
