mProX™ Human NR1H4 Stable Cell Line

Product Information

Target Protein

NR1H4

Target Family

Liver X Receptor

Target Protein Species

Human

Host Cell Type

HT29; CHO-K1; HEK293

Target Classification

Nuclear Receptor

Target Research Area

Digestive and Renal Research; Metabolic Research

Related Diseases

Cholestasis

Gene ID

9971

UniProt ID

Q96RI1

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The human NR1H4 gene encodes the bile acid receptor (BAR), often referred to as the farnesoid X receptor (FXR) or NR1H4. It is a nuclear receptor. The intestine and liver both exhibit significant levels of FXR expression. Natural FXR ligands include bile acids such as chenodeoxycholic acid. Like other nuclear receptors, FXR translocates to the cell nucleus upon activation, forms a dimer, and binds to DNA regions known as hormone response elements to either up- or down-regulate the production of specific genes. Suppression of cholesterol 7 alpha-hydroxylase (CYP7A1), the rate-limiting enzyme in the manufacture of bile acid from cholesterol, is one of the main effects of FXR activation. The customized NR1H4 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

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I can confidently say that the NR1H4 cell line is a fantastic tool. It has significantly improved the accuracy and precision of our experiments. Apr 25 2023

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chat James

This mouse NR1H4 KO cell line is the best choice for laboratories wishing to minimise troubleshooting time. Sep 22 2022

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FAQ

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Published Data

Fig.1 Generation of NR1H4 KO cell lines using CRISPR/CAS9 technology.

A 96-well plate was seeded with GFP-positive single cells, and the cells were then cultured until colonies could be seen. The expression of NR1H4 was detected by immunoblotting the colonies. It was determined that four KO cell line clones (#1-18, #1-20, #1-22, and #2-13) were NR1H4 KO. For additional analysis, one MOCK cell line clone and three of the KO cell line clones (#1-18, #1-20, and #1-22) were utilized.

Ref: Lee, Yun Jeong, et al. "The role of nuclear receptor subfamily 1 group H member 4 (NR1H4) in colon cancer cell survival through the regulation of c-Myc stability." Molecules and cells 43.5 (2020): 459.

Pubmed: 32299194

DOI: 10.14348/molcells.2020.0041

Research Highlights

According to the research, regulating NR1H4 activity in colon cancer cells may be a viable substitute strategy for treating cancer using MYC-targeting medications.
Lee, Yun Jeong, et al. "The role of nuclear receptor subfamily 1 group H member 4 (NR1H4) in colon cancer cell survival through the regulation of c-Myc stability." Molecules and cells 43.5 (2020): 459.
Pubmed: 32299194 DOI: 10.14348/molcells.2020.0041

The main cilia are microtubule-based cellular organelles that have been preserved throughout evolution. They sense metabolic state and connect the sensory system to cellular signaling pathways. Thus, autophagy, which is likewise controlled by nutrient-sensing transcription factors like PPARA (peroxisome proliferator activated receptor alpha) and NR1H4/FXR (nuclear receptor subfamily 1, group H, member 4) is assumed to be closely associated with ciliogenesis.
Liu, Zhi-qiang, et al. "Ciliogenesis is reciprocally regulated by PPARA and NR1H4/FXR through controlling autophagy in vitro and in vivo." Autophagy 14.6 (2018): 1011-1027.
Pubmed: 29771182 DOI: 10.1080/15548627.2018.1448326