mProX™ Human NOD1 Stable Cell Line
- Product Category:
- Membrane Protein Stable Cell Lines
- Subcategory:
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
Made to Order Inquiry
InquiryProduct Information
Product Properties
Protocols
Please visit our protocols page.
Customer Reviews
There are currently no Customer reviews or questions for mProX™ Human NOD1 Stable Cell Line (S01YF-1023-PY297). Click the button above to contact us or submit your feedback about this product.
Jordan Jones (Verified Customer)
Patrick Liam (Creative Biolabs Scientific Support)
Jordan Brown (Verified Customer)
Patrick Liam (Creative Biolabs Scientific Support)
Published Data
Fig.1 Reduced NOD1 expression and diminished IL-8 production in response to H. pylori are observed when NOD1 KD is implemented in AGS cells.
Untreated AGS control and AGS NOD1 KD cells were subjected to co-cultivation with H. pylori 251 for a duration of 6 hours. The assessment of NOD1 and CXCL8 mRNA expression was conducted via RT-PCR, with subsequent normalization relative to ACTB expression. Subsequently, cells underwent either no treatment (observed in lanes 1 and 3) or were induced with H. pylori 251 (evident in lanes 2 and 4).
Ref: Grubman, Alexandra, et al. "The innate immune molecule, NOD1, regulates direct killing of Helicobacter pylori by antimicrobial peptides." Cellular microbiology 12.5 (2010): 626-639.
Pubmed: 20039881
DOI: 10.1111/j.1462-5822.2009.01421.x
Research Highlights
L Dixon, Charneal. et al. "Multifaceted roles and regulation of nucleotide-binding oligomerization domain containing proteins." Frontiers in immunology, 2023.
The nucleotide-binding oligomerization domain-containing proteins, NOD1 and NOD2, are cytosolic receptors that identify dipeptides and tripeptides from peptidoglycan (PGN) in bacterial cell walls. Over the past 20 years, research has uncovered multiple functions for NODs in addition to recognizing PGN fragments, such as triggering an innate immune response against viruses, NOD-mediated autophagy, and causing inflammation through ER stress. Recent studies have also elucidated the control of NODs at cellular membranes to generate specific and balanced immune responses. This paper will discuss how NOD1 and NOD2 perceive microorganisms and cellular distress and explore the molecular mechanisms involved in their activation and signaling, with a focus on new findings and their impact on the development of inflammatory diseases.
L Dixon, Charneal. et al. "Multifaceted roles and regulation of nucleotide-binding oligomerization domain containing proteins." Frontiers in immunology, 2023.
Pubmed:
37869013
DOI:
10.3389/fimmu.2023.1242659
Marlene Schmid, Anna. et al. "Extracellular vesicles of the probiotic bacteria E. coli O83 activate innate immunity and prevent allergy in mice." Cell communication and signaling : CCS, 2023.
E. coli O83 (Colinfant Newborn), a Gram-negative (G-) probiotic bacterium, has been clinically used to reduce allergic sensitisation, but its effectiveness in treating allergic asthma has not been established. In this study, researchers examined the potential of EcO83-OMVs, secreted by G- bacteria like E. coli O83, as a non-invasive and convenient means of delivering the probiotic to the airways, the target of asthma prevention. The team investigated the ability of intranasally administered EcO83-OMVs to reduce allergic airway inflammation in mice. The results of this study are presented and discussed.
Marlene Schmid, Anna. et al. "Extracellular vesicles of the probiotic bacteria E. coli O83 activate innate immunity and prevent allergy in mice." Cell communication and signaling : CCS, 2023.
Pubmed:
37864211
DOI:
10.1186/s12964-023-01329-4
