mProX™ Human NIM1K Stable Cell Line
- Product Category:
- Membrane Protein Stable Cell Lines
- Subcategory:
- Kinase Cell Lines
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InquiryBased on this stable cell line, we also provide cell-based in vitro assays to evaluate the effects of your compounds or antibodies.
| Sub Cat | Product Name | Target Protein Species | Host Cell Type | Assay Types | Inquiry | Datasheet |
|---|---|---|---|---|---|---|
| S01YF-1222-KX405 | Magic™ Human NIM1K(MGC42105) in Vitro Assay | Human | Kinase Assay |
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Taylor Johnson (Verified Customer)
Patrick Liam (Creative Biolabs Scientific Support)
Casey Smith (Verified Customer)
Patrick Liam (Creative Biolabs Scientific Support)
Published Data
Fig.1 LKB1 regulation does not affect NIM1, as the same catalytic activity was observed when NIM1 was immunoprecipitated from both control HeLa cells and cells expressing catalytically active LKB1.
The determination of NIM1 activity derived from endogenous sources in the indicated HeLa cell lysates was carried out through the utilization of the AMARA peptide as substrate during immunoprecipitation.
Ref: Jaleel, Mahaboobi, et al. "Identification of the sucrose non-fermenting related kinase SNRK, as a novel LKB1 substrate." FEBS letters 579.6 (2005): 1417-1423.
Pubmed: 15733851
DOI: 10.1016/j.febslet.2005.01.042
Research Highlights
Illa, Satish Kumar et al. "Genome-Wide Scanning for Signatures of Selection Revealed the Putative Genomic Regions and Candidate Genes Controlling Milk Composition and Coat Color Traits in Sahiwal Cattle." Frontiers in genetics vol. 12 699422. 9 Jul. 2021.
In the context of evolutionary time scales, natural selection shapes genetic diversity and modifies genome architecture in organisms, leaving discernible markers at specific genomic positions that offer insights into protein-coding regions. Sahiwal cattle, a prized indicine breed adapted to tropical conditions with favorable milk attributes, prompted an investigation into potential selection signals within their genome using a medium-density genotyping INDUS chip. The analysis employed De-correlated Composite of Multiple Selection Signals (DCMS), integrating five distinct statistical measures, to identify signatures of selection in the Sahiwal genome. Subsequent gene annotations, Quantitative Trait Loci (QTL) enrichment assessments, and functional investigations pinpointed 117 genes influencing crucial economic traits. Significant QTL enrichments emerged on chromosomes BTA 1, 3, 6, 11, 20, and 21, with top QTLs on BTA 6, 20, and 23 linked to traits encompassing exterior attributes, health, milk production, and reproduction. Notable genes, such as PDGFRA, KIT, KDR, LEF, MAP3K1, HADH, CYP2U1, SGMS2, OSTC, MRPS30, NNT, CCL28, HMGCS1, NIM1K, ZNF131, CCDC152, GHR, ZNF469, NKX2-1, DENND1A, RNF144B, and PAPSS1, were associated with coat color, facial pigmentation, milk fat percentage, sperm membrane integrity, lactation persistency, milk yield, reproduction, and bovine tuberculosis susceptibility. Furthermore, hub genes like KIT, KDR, MAP3K1, and LEF played pivotal roles in coat color, facial pigmentation, and milk fat percentage. Gene enrichment analysis unveiled significant Gene Ontology (GO) terms related to breed-specific coat color and milk fat percentage. Ultimately, this study identified key candidate genes and potential genomic regions linked to economic traits in Sahiwal cattle using single nucleotide polymorphism data and the DCMS approach, shedding light on selection pressures favoring milk production, coat color, and tropical adaptability. This knowledge provides a foundation for unraveling the mechanisms underpinning these traits in Sahiwal cattle.
Illa, Satish Kumar et al. "Genome-Wide Scanning for Signatures of Selection Revealed the Putative Genomic Regions and Candidate Genes Controlling Milk Composition and Coat Color Traits in Sahiwal Cattle." Frontiers in genetics vol. 12 699422. 9 Jul. 2021.
Pubmed:
34306039
DOI:
10.3389/fgene.2021.699422
Faber, Siebren et al. "PDE6D Mediates Trafficking of Prenylated Proteins NIM1K and UBL3 to Primary Cilia." Cells vol. 12,2 312. 13 Jan. 2023.
PDE6D mutations disrupt the function of phosphodiesterase 6D (PDE6D), affecting the transport of prenylated proteins to the ciliary membrane and leading to Joubert Syndrome (JBTS22) in humans and retinal degeneration in mice. In their research, scientists purified PDE6D's prenylated cargo using affinity proteomics to gain insights into the disease mechanisms. They discovered a specific group of PDE6D-interacting proteins related to photoreceptor integrity, GTPase activity, nuclear import, and ubiquitination. Among these, novel ciliary cargo proteins were identified, including FAM219A, serine/threonine-protein kinase NIM1 (NIM1K), and ubiquitin-like protein 3 (UBL3). The study found that NIM1K and UBL3 localize within the cilium due to prenylation, with UBL3 also present in vesicle-like structures near the cilium's base. Affinity proteomics of UBL3 confirmed its strong interaction with PDE6D and its association with proteins governing small extracellular vesicles (sEVs) and ciliogenesis. Additionally, UBL3 was observed in specific photoreceptor cilium compartments in a prenylation-dependent manner, suggesting a potential role in protein sorting towards the photoreceptor outer segment and contributing to PDE6D-associated retinal degeneration.
Faber, Siebren et al. "PDE6D Mediates Trafficking of Prenylated Proteins NIM1K and UBL3 to Primary Cilia." Cells vol. 12,2 312. 13 Jan. 2023.
Pubmed:
36672247
DOI:
10.3390/cells12020312
