mProX™ Human MKNK1 Stable Cell Line

Huang, Tianyi. et al. "A tRF-5a fragment that regulates radiation resistance of colorectal cancer cells by targeting MKNK1." Journal of cellular and molecular medicine, 2023.
The efficacy of radiotherapy in treating colorectal cancer (CRC) is often hindered by radiation resistance. Although existing literature suggests a connection between tRNA-derived small RNAs (tsRNAs) and various cancers, there is a lack of studies examining the relationship between tsRNAs and cancer cell radiosensitivity. This study utilized tsRNA sequencing to identify differentially expressed tsRNAs in two CRC cells and their radioresistant counterparts. Ten tsRNAs showed significant differences in expression and were validated through qPCR. The target genes of tRF-16-7X9PN5D were predicted and verified through bioinformatics, dual-luciferase reporter gene assay, and western blotting analyses. Wound healing, colony formation, transwell invasion, and CCK-8 assays were performed to assess the effects of tRF-16-7X9PN5D on cell function and radiosensitivity. Our findings demonstrated that tRF-16-7X9PN5D was downregulated in radioresistant CRC cells and could promote cell proliferation, migration, invasion, and radioresistance by targeting MKNK1. Furthermore, tRF-16-7X9PN5D could regulate eIF4E phosphorylation through MKNK1. This study highlights the crucial regulatory role of tRF-16-7X9PN5D in CRC radiation resistance and suggests a potential new pathway for regulating CRC radiosensitivity.
Huang, Tianyi. et al. "A tRF-5a fragment that regulates radiation resistance of colorectal cancer cells by targeting MKNK1." Journal of cellular and molecular medicine, 2023.
Pubmed: 37864471   DOI: 10.1111/jcmm.17982

Pal, Ankit. et al. "Circulatory extracellular vesicle derived miR-195-5p promotes cellular apoptosis and suppresses cell proliferation in the buffalo endometrial primary cell culture." Scientific reports, 2023.
In pregnant animals, communication between the mother and conceptus is mediated by extracellular vesicles (EVs) that transport various biomolecules such as nucleic acids, proteins, and lipids. Researchers conducted a study to investigate the presence and function of pregnancy-specific miRNAs in EVs in the systemic circulation of Bubalus bubalis (water buffalo). EVs were isolated from blood plasma and characterized using different techniques. The expression of one specific EV-derived miRNA, miR-195-5p, was significantly higher in pregnant animals on day 19 post artificial insemination (AI), suggesting its involvement in maternal-conceptus cross-talk during the implantation window. Analysis of miR-195-5p target genes revealed their possible role in regulating cell proliferation and was further confirmed by transfecting cultured cells with miR-195-5p mimic, resulting in downregulation of target genes and promoting apoptosis and inhibiting proliferation. These findings demonstrate the functional significance of EV-derived miRNA in modifying endometrial epithelial cell behavior and promoting cell death during early pregnancy in buffaloes. The unique presence of miR-195-5p in the circulatory EVs may serve as a potential biomarker for early pregnancy detection in buffaloes as early as day 19 post-AI.
Pal, Ankit. et al. "Circulatory extracellular vesicle derived miR-195-5p promotes cellular apoptosis and suppresses cell proliferation in the buffalo endometrial primary cell culture." Scientific reports, 2023.
Pubmed: 37794118   DOI: 10.1038/s41598-023-43530-y