mProX™ Human MINK1 Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-1122-KX1221	Magic™ Human MINK(ZC3) in Vitro Assay	Human		Kinase Assay

Product Information

Target Family

Kinases/Enzyme

Target Protein Species

Human

Host Cell Type

HEK293;CHO-K1;THP-1

Target Classification

Kinase Cell Lines

Target Research Area

CNS Research

Related Diseases

Alzheimer Disease 15; Shipyard Eye

Gene ID

Human:50488

UniProt ID

Human:Q8N4C8

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

MINK1 is a serine/threonine kinase that has been found to have several applications in different fields. In breast cancer, high levels of MINK1 expression have been associated with shorter overall survival of patients, suggesting its possible role in breast cancer development and prognosis. In the context of congenital heart disease (CHD), MINK1 has been identified as a crucial regulator of cell fate determination in the Spemann Organizer, highlighting a potential molecular mechanism connecting CHD and MINK1. MINK1 has also been implicated in Alzheimer's disease risk, as variants in the MINK1 gene have been identified as risk factors for the disease in African American individuals. Additionally, in Th17-dominant asthma, MINK1 is regulated by methyl-CpG binding domain protein 2 (MBD2) and plays a role in the differentiation of Th17 cells, suggesting its potential as a therapeutic target for this type of asthma. Lastly, in the kidney, vasopressin regulates protein phosphorylation, including MINK1, in the collecting duct, indicating its involvement in the control of water excretion. Overall, the research suggests that MINK1 has diverse applications in various diseases and biological processes.

Protocols

Please visit our protocols page.

Customer Reviews

There are currently no Customer reviews or questions for mProX™ Human MINK1 Stable Cell Line (S01YF-1023-PY4). Click the button above to contact us or submit your feedback about this product.

FAQ

chat Taylor Smith (Verified Customer)

How does MINK1 contribute to inflammasome activation? Sep 10 2021

chat Patrick Liam (Creative Biolabs Scientific Support)

MINK1, a kinase, primes the activation of the NLRP3 inflammasome in macrophages, suggesting its involvement in inflammatory responses. Sep 10 2021

chat Taylor Davis (Verified Customer)

What is the role of MINK1 in drug resistance? May 26 2020

chat Patrick Liam (Creative Biolabs Scientific Support)

MINK1 has been identified as a druggable player to rewire 5FU-resistance in oral squamous cell carcinoma (OSCC), highlighting its therapeutic potential. May 26 2020

Published Data

Fig.1 Overepxression of MINK1 in THP-1 cells.

MINK1 expression was increased in THP-1 cells after transfection of pcDNA3.1/MINK1. RT-qPCR was used to detect the efficiency of MINK1 overexpression.

Ref: Zhang, Jihui, Hongwei Lei, and Xiu Li. "LncRNA SNHG14 contributes to proinflammatory cytokine production in rheumatoid arthritis via the regulation of the miR-17-5p/MINK1-JNK pathway." Environmental toxicology 36.12 (2021): 2484-2492.

Pubmed: 34529319

DOI: 10.1002/tox.23361

Research Highlights

Xinyu Li, Amber. et al. "Striatins and STRIPAK complex partners in clinical outcomes of patients with breast cancer and responses to drug treatment." Chinese journal of cancer research = Chung-kuo yen cheng yen chiu, 2023.
The Striatins (STRNs) family, consisting of three multi-domain scaffolding proteins, is an integral component of the striatins interacting phosphatase and kinase (STRIPAK) complex. While the involvement of the STRIPAK complex in cancer has gained recognition in recent times, its potential clinical impact in breast cancer remains to be fully determined.
Xinyu Li, Amber. et al. "Striatins and STRIPAK complex partners in clinical outcomes of patients with breast cancer and responses to drug treatment." Chinese journal of cancer research = Chung-kuo yen cheng yen chiu, 2023.
Pubmed: 37691891 DOI: 10.21147/j.issn.1000-9604.2023.04.04

Colleluori, Vaughn; K, Mustafa. "Mink1 regulates spemann organizer cell fate in the xenopus gastrula via Hmga2." Developmental biology, 2023.
Congenital Heart Disease (CHD) is a prevalent birth defect and the primary cause of infant mortality. Despite its frequency, the underlying molecular mechanisms of CHD are largely unknown. Recent sequencing studies have identified potential CHD candidate genes, including MINK1, a serine/threonine kinase. However, the exact connection between CHD and MINK1 remains unclear. Through research, it was found that mink1 plays a crucial role in heart development by regulating the body's left-right patterning. This is achieved through its regulation of canonical Wnt signaling, which dictates the cell fates of the Spemann Organizer and the Left-Right Organizer, a ciliated structure essential for breaking bilateral symmetry in the vertebrate embryo. Further investigations revealed that a high mobility group architectural transcription factor called Hmga2 is a direct target of Mink1. It was discovered that Hmga2 is essential for the proper functioning of Spemann's Organizer and can even induce cell fates of Spemann's Organizer in the absence of Œ≤-catenin, a crucial mediator of the Wnt signaling pathway. In essence, this study uncovers the role of Hmga2, downstream of Mink1, in regulating the function of both the Spemann Organizer and the Left-Right Organizer, providing a possible explanation for the development of CHD.
Colleluori, Vaughn; K, Mustafa. "Mink1 regulates spemann organizer cell fate in the xenopus gastrula via Hmga2." Developmental biology, 2023.
Pubmed: 36572140 DOI: 10.1016/j.ydbio.2022.11.010