mProX™ Human MELK Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-1122-KX1213	Magic™ Human MELK in Vitro Assay	Human		Kinase Assay

Product Information

Target Family

Kinases/Enzyme

Target Protein Species

Human

Host Cell Type

HEK293;CHO-K1;NGP;IMR-32;SH-SY5Y;SK-N-AS

Target Classification

Kinase Cell Lines

Gene ID

Human:9833

UniProt ID

Human:Q14680

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

MELK (maternal embryonic leucine zipper kinase) has various applications in different fields. In pediatric research, MELK is used to develop reference values for parameters of phosphate homeostasis in children. The study provides reference percentiles for key laboratory parameters of phosphate homeostasis that can aid in the interpretation of test results in children and adolescents. In the context of diffuse high-grade gliomas, MELK is studied in comparison to 2D cell cultures and tissue strains to understand its role in tumor progression and characteristics. It is found that 3D spheroids, in which MELK is expressed, more closely mimic tumor tissue compared to 2D cell cultures. In rheumatoid arthritis, MELK is identified as one of the hub genes that could serve as a potential therapeutic target for the disease. In nasopharyngeal carcinoma, MELK is shown to play a role in immune escape, making it a potential target for suppressing the immune evasion of tumor cells. Finally, in colitis-associated colon cancer, MELK is explored for its potential role in reducing initiation and preventing the development of colon cancer associated with inflammatory bowel disease. These studies highlight the diverse applications of MELK in different areas of research and its potential as a target for therapeutic interventions.

Protocols

Please visit our protocols page.

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FAQ

chat Sarah (Verified Customer)

What is the role of MELK in cellular processes? Jan 01 2021

chat Patrick Liam (Creative Biolabs Scientific Support)

Maternal embryonic leucine zipper kinase (MELK) is involved in regulating various cellular events including stem cell renewal, cell cycle progression, and pre-mRNA splicing. Jan 01 2021

chat Joe (Verified Customer)

How is MELK associated with cancer? Jan 01 2022

chat Patrick Liam (Creative Biolabs Scientific Support)

Aberrant expression of MELK is linked to tumorigenesis and malignant progression in various cancers, and its expression is higher in numerous cancer cells compared to their normal counterparts. Jan 01 2022

Published Data

Fig.1 MELK knockdown inhibits Rb phosphorylation in all NB cell lines considerably.

Effects of MELK knockdown on p-Rb levels in NB cell lines were shown by immunoblotting assay. These results confirm that MELK plays a oncogenic role via regulating the activation of Rb protein in NB.

Ref: Guan, Shan, et al. "MELK is a novel therapeutic target in high-risk neuroblastoma." Oncotarget 9.2 (2018): 2591.

Pubmed: 29416794

DOI: 10.18632/oncotarget.23515

Research Highlights

Pott, Veronika. et al. "LMS-based pediatric reference values for parameters of phosphate homeostasis in the HARP cohort." The Journal of clinical endocrinology and metabolism, 2023.
The evaluation of phosphate homeostasis presents difficulties in children due to the significant fluctuations in laboratory indicators during their growth and development. Moreover, there is a lack of suitable reference values to accurately assess phosphate levels in this population. These challenges may hinder proper diagnosis and management of phosphate-related disorders in children. Further research and establishment of reliable reference ranges are necessary to address these obstacles and improve the accuracy of evaluating phosphate homeostasis in pediatric patients.
Pott, Veronika. et al. "LMS-based pediatric reference values for parameters of phosphate homeostasis in the HARP cohort." The Journal of clinical endocrinology and metabolism, 2023.
Pubmed: 37850343 DOI: 10.1210/clinem/dgad597

V Arutyunyan, I. et al. "Gene Expression Profile of 3D Spheroids in Comparison with 2D Cell Cultures and Tissue Strains of Diffuse High-Grade Gliomas." Bulletin of experimental biology and medicine, 2023.
The use of relevant, accessible, and easily reproducible preclinical models is crucial for effective therapeutic development for diffuse gliomas. This study compared the gene expression profiles of 3D spheroids, 2D cell cultures, and tissue strains of diffuse high-grade gliomas. Through real-time PCR, the expression of 10 important genes (Gfap, Cd44, Pten, S100b, Vegfa, Hif1a, Sox2, Melk, Gdnf, and Mgmt) was evaluated, which regulate glioma progression, cell proliferation, adhesion, invasion, plasticity, apoptosis, DNA repair, and recruitment of tumor-associated cells. Results showed that 3D spheroids showed closer similarity to tumor tissue strains in terms of Gfap, Cd44, and Pten expression. However, 3D spheroids did not significantly differ from 2D cell cultures in expression levels of Hif1a and Sox2. Furthermore, S100b and Vegfa were expressed at higher levels in the 3D spheroid model compared to other models, while expression levels of Melk, Gdnf, and Mgmt genes varied. Overall, the 3D spheroid model is a better representation of tumor tissue compared to 2D cell culture, but its small size may limit its ability to replicate hypoxia, apoptosis, and necrosis in tumor tissue.
V Arutyunyan, I. et al. "Gene Expression Profile of 3D Spheroids in Comparison with 2D Cell Cultures and Tissue Strains of Diffuse High-Grade Gliomas." Bulletin of experimental biology and medicine, 2023.
Pubmed: 37770789 DOI: 10.1007/s10517-023-05906-y