mProX™ Human MARK3 Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-1122-KX1211	Magic™ Human MARK3 in Vitro Assay	Human		Kinase Assay

Product Information

Target Protein

MARK3

Target Family

Kinases/Enzyme Drug Discovery Assays and Products

Target Protein Species

Human

Host Cell Type

CHO-K1; HEK293

Target Classification

Kinase Cell Lines

Target Research Area

Ocular Research; Pigmentation Research; Reproductive Research

Related Diseases

Visual Impairment And Progressive Phthisis Bulbi and Peutz-Jeghers Syndrome. Among its related pathways are Signaling downstream of RAS mutants and RAF/MAP kinase cascade

Gene ID

4140

UniProt ID

P27448

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

A member of the MARK family, microtubule affinity-regulating kinase 3 (MARK3) controls the differentiation of ciliated cells, osteoclasts, and the cell cycle, among other critical pathways. Given that MARK3 has three domains-a ubiquitin-associated domain, a C-terminal kinase-associated domain, and an N-terminal serine/threonine kinase domain-all of which carry out various regulatory tasks, it is critical to comprehend how their actions are regulated. These include interactions with other proteins and scaffolding, as well as post-translational modifications like phosphorylation. According to recent research, MARK3 may be involved in a number of clinical disorders, such as cancer, osteoporosis, and congenital blepharophimosis syndrome. The customized MARK3 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

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With the mouse MARK3 KO cell line, I can now complete my experiments in a fraction of the time. Aug 05 2023

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The MARK3 cell line is a cost-effective solution. Oct 07 2021

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FAQ

Any questions about our products? Please visit our frequently asked questions page.

Published Data

Fig.1 Phosphatase treatment of MARK3.

In HEK 293 cells, wild-type GST-MARK3 was produced, purified on glutathione-Sepharose, and either treated with PP1γ in the presence of microcystin-LR or not. Following immunoprecipitation with αHA antibodies, the HA-tagged GST-MARK3 was cleaned and subjected to western blot analysis for total protein content.

Ref: Göransson, Olga, et al. "Regulation of the polarity kinases PAR-1/MARK by 14-3-3 interaction and phosphorylation." Journal of cell science 119.19 (2006): 4059-4070.

Pubmed: 16968750

DOI: 10.1242/jcs.03097

Research Highlights

Given that MARK3/par-1 function is evolutionarily conserved in eye formation and that the detected mutation in MARK3 likely causes structural defects during eye development and visual impairment in humans, this finding establishes a new gene-disease relationship.
Ansar, Muhammad, et al. "Visual impairment and progressive phthisis bulbi caused by recessive pathogenic variant in MARK3." Human molecular genetics 27.15 (2018): 2703-2711.
Pubmed: 29771303 DOI: 10.1093/hmg/ddy180

Through the use of ARHGEF2, this work has discovered a regulatory switch regulated by MARK3, which links microtubules to the actin cytoskeleton to establish epithelial cell polarity.
Sandí, María-José, et al. "MARK3-mediated phosphorylation of ARHGEF2 couples microtubules to the actin cytoskeleton to establish cell polarity." Science signaling 10.503 (2017): eaan3286.
Pubmed: 29089450 DOI: 10.1126/scisignal.aan3286