mProX™ Human MARK2 Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-1122-KX1210	Magic™ Human MARK2 in Vitro Assay	Human		Kinase Assay

Product Information

Target Protein

MARK2

Target Family

Kinases/Enzyme Drug Discovery Assays and Products

Target Protein Species

Human

Host Cell Type

U2-OS; CHO-K1; HEK293

Target Classification

Kinase Cell Lines

Related Diseases

Among its related pathways are Sertoli-Sertoli Cell Junction Dynamics and Complement cascade

Gene ID

2011

UniProt ID

Q7KZI7

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

Kinase for serine/threonine proteins The MARK2 gene in humans codes for the enzyme MARK2. A small family of ser/thr protein kinases called EMK (ELKL Motif Kinase) is implicated in the regulation of cancer, microtubule stability, and cell polarity. Two isoforms of the human ser/thr protein kinase EMK1 have been encoded by a number of cDNA clones that have been identified. A 162-bp alternative exon that resulted in two forms-one with the exon and the other without-was what distinguished these isoforms. It was discovered that several chosen cell lines and tissue samples coexpressed both types. It has been demonstrated that a single mRNA that is widely expressed encodes the human EMK1. The customized MARK2 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat John

I have been using the MARK2 KD cell line for my research, and it has consistently provided reliable results. Aug 10 2022

chat Verified Customer

chat Thomas

The specificity and reproducibility of this MARK2 overexpression cell line are impressive. Apr 22 2020

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FAQ

Any questions about our products? Please visit our frequently asked questions page.

Published Data

Fig.1 MARK2 regulates MT growth dynamics and orientation downstream of Rac1.

Lysates from U2-OS cells transfected with non-targeting siRNA pool (lane 4), MARK2-shRNA (lane 2), control shRNA (lane 1), MARK2-shRNA (lane 2), and MARK2-shRNA and shRNA-resistant GFP-MARK2 (lane 3).

Ref: Nishimura, Yukako, et al. "Automated screening of microtubule growth dynamics identifies MARK2 as a regulator of leading edge microtubules downstream of Rac1 in migrating cells." PloS one 7.7 (2012): e41413.

Pubmed: 22848487

DOI: 10.1371/journal.pone.0041413

Research Highlights

The information showed that MARK2/4 promotes metabolic reprogramming in NSCLC cells, which is how it causes cancer. As a result, MARK2/4 may be a viable target for lung cancer treatment.
Natarajan, Sathan Raj, Lavanya Ponnusamy, and Ravi Manoharan. "MARK2/4 promotes Warburg effect and cell growth in non-small cell lung carcinoma through the AMPKα1/mTOR/HIF-1α signaling pathway." Biochimica et Biophysica Acta (BBA)-Molecular Cell Research 1869.7 (2022): 119242.
Pubmed: 35192892 DOI: 10.1016/j.bbamcr.2022.119242

In unperturbed mitosis and in response to antitubulin chemotherapy, MARK2 is phosphorylated by CDK1. MARK2 requires phosphorylation to control the course of mitosis and the cytotoxicity of paclitaxel in PDAC cells.
Zeng, Yongji, et al. "MARK2 regulates chemotherapeutic responses through class IIa HDAC-YAP axis in pancreatic cancer." Oncogene 41.31 (2022): 3859-3875.
Pubmed: 35780183 DOI: 10.1038/s41388-022-02399-3