mProX™ Human MAPKAPK3 Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-1122-KX1207	Magic™ Human MAPKAPK3 in Vitro Assay	Human		Kinase Assay

Product Information

Target Protein

MAPKAPK3

Target Family

Kinases/Enzyme Drug Discovery Assays and Products

Target Protein Species

Human

Host Cell Type

CHO-K1; HEK293

Target Classification

Kinase Cell Lines

Target Research Area

Inflammation Research; Reproductive Research

Related Diseases

Macular Dystrophy, Patterned, 3 and Bacteremia 2. Among its related pathways are MyD88 dependent cascade initiated on endosome and Signalling to ERKs

Gene ID

7867

UniProt ID

Q16644

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The human MAPKAPK3 gene encodes the enzyme MAP kinase-activated protein kinase 3. A member of the Ser/Thr protein kinase family is encoded by this gene. Mitogen-activated protein kinase (MAP kinase)-activated protein kinase is the function of this kinase. Extracellular signal-regulated kinases (ERKs), another name for MAP kinases, serve as a hub for a variety of biochemical signals. It has been demonstrated that growth inducers and cell stress stimuli activate this kinase. The ability of ERK, p38 MAP kinase, and Jun N-terminal kinase to phosphorylate and activate this kinase was shown in in vitro experiments, suggesting that this kinase functions as an integrative element of signaling in both mitogen and stress responses. The customized MAPKAPK3 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

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The human MAPKAPK3 cell line's ease of use and reproducibility have significantly expedited our experiments. Aug 17 2020

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The consistent performance of MAPKAPK3 cell line have significantly boosted the quality of our data. Dec 13 2022

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FAQ

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Published Data

Fig.1 Analysis of the expression of ACVR2B, PIM1, and MAPKAPK3 in cell lines and tumoral tissue.

Using tubulin as a reference, commercial antibodies against ACRV2B, PIM1, and MAPKAPK3 were used for Western blot analysis. After being separated by 10% SDS-PAGE and transferred to nitrocellulose membranes, 50 g of cell extracts from six distinct CRC cell lines and five controls were used.

Ref: Babel, Ingrid, et al. "Identification of tumor-associated autoantigens for the diagnosis of colorectal cancer in serum using high density protein microarrays." Molecular & cellular proteomics 8.10 (2009): 2382-2395.

Pubmed: 19638618

DOI: 10.1074/mcp.M800596-MCP200

Research Highlights

These results identify MK2/MK3 as essential stress-responsive kinases that stimulate autophagy via Beclin 1 S90 phosphorylation. They further show that BCL2 suppresses the autophagy function of Beclin 1 by blocking MK2/3-dependent Beclin 1 S90 phosphorylation.
Wei, Yongjie, et al. "The stress-responsive kinases MAPKAPK2/MAPKAPK3 activate starvation-induced autophagy through Beclin 1 phosphorylation." Elife 4 (2015): e05289.
Pubmed: 25693418 DOI: 10.7554/eLife.05289