mProX™ Human MAPK15 Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-1122-KX1134	Magic™ Human Erk7(MAPK15) in Vitro Assay	Human		Kinase Assay

Product Information

Target Protein

MAPK15

Target Family

Kinases/Enzyme Drug Discovery Assays and Products

Target Protein Species

Human

Host Cell Type

RPE1; CHO-K1; HEK293

Target Classification

Kinase Cell Lines

Target Research Area

Cancer Research

Related Diseases

Hemangioma Of Subcutaneous Tissue. Among its related pathways are VEGF Signaling Pathway and Death Receptor Signaling

Gene ID

225689

UniProt ID

Q8TD08

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

Humans have an enzyme called mitogen-activated protein kinase 15, or MAPK15, ERK7, or ERK8. The MAPK15 gene encodes this enzyme. Many species, such as P. troglodytes, B. taurus, M. musculus, R. norvegicus, D. rerio, D. melanogaster, C. elegans, and X. laevis, have conserved MAPK15. This gene codes for a protein that belongs to the family of mitogen-activated protein kinases, or MAPs. Extracellular signal-regulated kinases (ERKs), another name for MAP kinases, are engaged in signaling cascades that control several cellular functions such as transcriptional regulation, differentiation, and proliferation. Although ERK7 and ERK8, which are other names for MAPK15, have a 69% amino acid sequence similarity, at least one study indicates that the two are different proteins. The customized MAPK15 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

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The MAPK15 cell line proved to be an invaluable tool in my experiments. Its excellent performance and reproducibility greatly contributed to the success of my research project. Apr 11 2023

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chat Laura

The MAPK15 cell line I purchased was incredibly effective in my research. Jun 25 2020

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FAQ

Any questions about our products? Please visit our frequently asked questions page.

Published Data

Fig.1 MAPK15 controls primary ciliogenesis in a kinase-dependent fashion.

After being transfected with either scrambled siRNA (siSCR, negative control) or MAPK15 siRNA (siMAPK15), RPE1 cells were cultured for a further 48 hours in either full medium (FM) or starvation media (starvation). After that, the cells were fixed and examined using immunofluorescence.

Ref: Pietrobono, Silvia, et al. "MAPK15 controls hedgehog signaling in medulloblastoma cells by regulating primary ciliogenesis." Cancers 13.19 (2021): 4903.

Pubmed: 34638386

DOI: 10.3390/cancers13194903

Research Highlights

The kinase MAPK15 has been shown to be a potential therapeutic target in a variety of age-associated human diseases. It also protects primary human airway epithelial cells from senescence, establishing a new specific role for MAPK15 in controlling mitochondrial fitness by efficiently disposing of old and damaged organelles.
Franci, Lorenzo, et al. "MAPK15 protects from oxidative stress-dependent cellular senescence by inducing the mitophagic process." Aging Cell 21.7 (2022): e13620.
Pubmed: 35642724 DOI: 10.1111/acel.13620

There is a new "druggable" connection between BCR-ABL1 and human CML as depletion of endogenous MAPK15 expression suppressed BCR-ABL1-dependent cell proliferation in vitro and tumor growth in vivo.
Colecchia, David, et al. "MAPK15 mediates BCR-ABL1-induced autophagy and regulates oncogene-dependent cell proliferation and tumor formation." Autophagy 11.10 (2015): 1790-1802.
Pubmed: 26291129 DOI: 10.1080/15548627.2015.1084454