mProX™ Human MAPK1 Stable Cell Line
- Product Category:
- Membrane Protein Stable Cell Lines
- Subcategory:
- Kinase Cell Lines
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Patrick Liam (Creative Biolabs Scientific Support)
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Patrick Liam (Creative Biolabs Scientific Support)
Published Data
Fig.1 Confirmation of the Kasumi-1 and SKNO-1 cell lines' microarray data through quantitative real-time polymerase chain reaction (qRT-PCR) analysis was successfully achieved.
In triplicate experiments, we assessed microarray signal intensity and quantified MAPK1 and RUNX1-RUNX1T1 mRNA copy numbers, which were subsequently normalized to B2M expression. These findings are presented as means along with error bars representing standard deviations. For reference, we used siRNA-MAPK1 (siMAPK1) and siRNA-RUNX1-RUNX1T1 (siRR).
Ref: Bashanfer, Salem Ali Al‑Salem, et al. "Disruption of MAPK1 expression in the ERK signalling pathway and the RUNX1‑RUNX1T1 fusion gene attenuate the differentiation and proliferation and induces the growth arrest in t (8; 21) leukaemia cells." Oncology reports 41.3 (2019): 2027-2040.
Pubmed: 30569130
DOI: 10.3892/or.2018.6926
Research Highlights
Xia, Yong. et al. "Proteomics, Transcriptomics, and Phosphoproteomics Reveal the Mechanism of Talaroconvolutin-A Suppressing Bladder Cancer via Blocking Cell Cycle and Triggering Ferroptosis." Molecular & cellular proteomics : MCP, 2023.
Talaroconvolutin-A (TalaA) is a compound found in the endophytic fungus T. convolutispora, which is commonly found in the Chinese herbal medicine, Panax notoginseng. The potential anti-cancer properties of TalaA in bladder cancer have yet to be fully understood. Researchers conducted a study to explore the anticancer effects and pharmacological mechanism of TalaA from a molecular perspective. In-vitro analysis was performed using various assays and techniques, including CCK8, EdU staining, crystal violet staining, flow cytometry, living/dead cell staining, and western blotting. In-vivo experiments were conducted through xenograft tumor implantation. Results revealed that TalaA effectively inhibited the proliferation, DNA replication, and colony formation in bladder cancer cells in a dose-dependent manner, with an IC50 value of 1.3μg/ml. High-throughput omics detection, including proteomics, transcriptomics, and phosphoproteomics, was also used to analyze changes in the protein and mRNA profile, as well as protein phosphorylation. Additional evaluations were conducted through hematoxylin and eosin staining, immunohistochemistry staining, and pathological analysis.
Xia, Yong. et al. "Proteomics, Transcriptomics, and Phosphoproteomics Reveal the Mechanism of Talaroconvolutin-A Suppressing Bladder Cancer via Blocking Cell Cycle and Triggering Ferroptosis." Molecular & cellular proteomics : MCP, 2023.
Pubmed:
37866481
DOI:
10.1016/j.mcpro.2023.100672
Wang, ChunPeng. et al. "Network pharmacology-based strategy to investigate the effect and mechanism of α-solanine against glioma." BMC complementary medicine and therapies, 2023.
In this study, researchers delved into the potential anti-tumor properties of α-solanine, a bioactive compound derived from the traditional Chinese herb Solanum nigrum L, particularly focusing on its effectiveness against gliomas and the associated mechanisms. Employing network pharmacology, molecular docking, and molecular biology experiments, they identified 78 shared targets between α-solanine and glioma-related targets. Subsequently, 11 crucial hub genes were pinpointed, and their involvement in pathways such as MAP kinase activity and PI3K-Akt was revealed. Notably, α-solanine demonstrated its ability to impede glioma cell proliferation and migration while fostering apoptosis. The study also spotlighted STAT1 as a key player in α-solanine's impact on glioma prognosis, providing promising prospects for novel clinical anti-glioma treatments.
Wang, ChunPeng. et al. "Network pharmacology-based strategy to investigate the effect and mechanism of α-solanine against glioma." BMC complementary medicine and therapies, 2023.
Pubmed:
37865727
DOI:
10.1186/s12906-023-04215-1
