mProX™ Human MAP3K9 Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-1122-KX1222	Magic™ Human MLK1(MAP3K9) in Vitro Assay	Human		Kinase Assay

Product Information

Target Family

Kinases/Enzyme

Target Protein Species

Human

Host Cell Type

HEK293;CHO-K1;BxPC-3;PANC-1;PaTu-8988

Target Classification

Kinase Cell Lines

Target Research Area

Cancer Research;CNS Research

Related Diseases

Retroperitoneal Neuroblastoma; Retroperitoneum Carcinoma

Gene ID

Human:4293

UniProt ID

Human:P80192

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

MAP3K9, also known as mixed lineage kinase 9, is involved in various biological processes and disease pathways. In the context of thyroid cancer, changes in antioxidant status and DNA repair capacity were found to be associated with molecular alterations in malignant thyroid tissue, and MAP3K9 was found to be one of the mutated genes in both benign thyroid lesions and papillary thyroid cancer (PTC) lesions. This suggests that MAP3K9 may play a role in the transformation of benign thyroid lesions to PTC through the MAPK signaling pathway. In the context of ovarian function, MAP3K9 was identified as a target of microRNA-144-3p, which protects against chemotherapy-induced apoptosis of ovarian granulosa cells and activation of primordial follicles. This suggests that MAP3K9 may be involved in the regulation of ovarian function and could be a potential therapeutic target for preserving ovarian reserve. In the context of honeybee development, low temperature stress during the early pupal stage was found to affect the MAPK signaling pathway, including MAP3K9, as well as pathways related to the nervous system. This suggests that MAP3K9 may contribute to the development and learning ability of adult honeybees. In the context of peri-hilar cholangiocarcinoma, a targetable mutation in MAP3K9 was identified, and pathway analysis revealed its involvement in immunological pathways, including innate and adaptive immune responses. This suggests that MAP3K9 may be a potential target for therapeutic intervention in peri-hilar cholangiocarcinoma. Overall, MAP3K9 has implications in oxidative stress, DNA repair, ovarian function, honeybee development, and cholangiocarcinoma, highlighting its diverse applications in different biological processes and diseases.

Protocols

Please visit our protocols page.

Customer Reviews

There are currently no Customer reviews or questions for mProX™ Human MAP3K9 Stable Cell Line (S01YF-1023-PY5). Click the button above to contact us or submit your feedback about this product.

FAQ

chat Alex Smith (Verified Customer)

What is the impact of MAP3K9 mutations in cancer? Dec 13 2020

chat Patrick Liam (Creative Biolabs Scientific Support)

Mutations in MAP3K9, such as the W333X mutation, can result in the production of a truncated non-functional protein, affecting kinase activity and potentially influencing cancer development. Dec 13 2020

chat Jordan Davis (Verified Customer)

Does MAP3K9 have a direct enzymatic role in cancer cells? Mar 08 2022

chat Patrick Liam (Creative Biolabs Scientific Support)

MAP3K9 has been shown to directly phosphorylate MEK in kinase assays, indicating its active role in the MAP kinase signal transduction pathway and potential as a therapeutic target. Mar 08 2022

Published Data

Fig.1 MAP3K9 promoted cell proliferation in PC cells.

Following transfection with MAP3K9 cDNA in BxPC-3 cells or MAP3K9 siRNA in PANC-1 and PaTu-8988 cells, an MTT assay was conducted, and error bars were incorporated to represent standard errors. Significance, denoted as *p < 0.05, was observed in comparison to the control group.

Ref: Xia, Jun, et al. "miR-7 suppresses tumor progression by directly targeting MAP3K9 in pancreatic cancer." Molecular Therapy-Nucleic Acids 13 (2018): 121-132.

Pubmed: 30290304

DOI: 10.1016/j.omtn.2018.08.012

Research Highlights

Hong Eng, Zing. et al. "Changes in antioxidant status and DNA repair capacity are corroborated with molecular alterations in malignant thyroid tissue of patients with papillary thyroid cancer." Frontiers in molecular biosciences, 2023.
This study examines the impact of physical activity on cognitive function among elderly individuals. The research aims to understand how engaging in physical activity can improve cognitive functioning in older adults. A literature review will be conducted to gather relevant information and studies to support the research. Data will be collected through questionnaires and cognitive tests, and conclusions will be drawn based on the results. The findings of this study have the potential to provide new insights into the benefits of physical activity for cognitive health in the aging population.
Hong Eng, Zing. et al. "Changes in antioxidant status and DNA repair capacity are corroborated with molecular alterations in malignant thyroid tissue of patients with papillary thyroid cancer." Frontiers in molecular biosciences, 2023.
Pubmed: 37692064 DOI: 10.3389/fmolb.2023.1237548

Liu, Meng. et al. "MicroRNA-144-3p protects against chemotherapy-induced apoptosis of ovarian granulosa cells and activation of primordial follicles by targeting MAP3K9." European journal of medical research, 2023.
Premature ovarian failure (POF), characterized by hormonal imbalance and infertility before the age of 40, is a condition often caused by chemotherapy. However, the exact mechanisms behind chemotherapy-induced ovarian damage are still unknown. This research examined a POF mouse model treated with cisplatin, a common chemotherapy agent, and found increased cell death in granulosa cells (GCs) and abnormal activation of primordial follicles. Additionally, the study identified a significant decrease in miR-144-3p and an increase in MAP3K9 expression in the ovaries of POF mice and human ovarian granulosa cells exposed to cisplatin. Further investigation revealed that miR-144-3p acts as a protective agent against cisplatin-induced apoptosis in GCs by targeting MAP3K9 and suppressing the activity of the p38MAPK pathway. It also prevents premature depletion of primordial follicles by targeting Map3k9 and inhibiting the activation of the PI3K/AKT pathway. These findings highlight the potential of miR-144-3p as a biomarker for ovarian reserve and shed light on the epigenetic regulatory role in POF development.
Liu, Meng. et al. "MicroRNA-144-3p protects against chemotherapy-induced apoptosis of ovarian granulosa cells and activation of primordial follicles by targeting MAP3K9." European journal of medical research, 2023.
Pubmed: 37537658 DOI: 10.1186/s40001-023-01231-2