mProX™ Human MAP3K4 Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-1122-KX1204	Magic™ Human MAP3K4 in Vitro Assay	Human		Kinase Assay

Product Information

Target Protein

MAP3K4

Target Family

Kinases/Enzyme Drug Discovery Assays and Products

Target Protein Species

Human

Host Cell Type

MCF-7; CHO-K1; HEK293

Target Classification

Kinase Cell Lines

Target Research Area

Cancer Research

Related Diseases

Breast Neuroendocrine Neoplasm. Among its related pathways are NGF Pathway and NF-KappaB Family Pathway

Gene ID

4216

UniProt ID

Q96B97

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The MAP3K4 gene in humans encodes the enzyme mitogen-activated protein kinase kinase 4. A conserved cascade of three protein kinases forms the basic core of every mitogen-activated protein kinase (MAPK) pathway: an active MAPK kinase kinase (MAPKKK) phosphorylates and activates a particular MAPK kinase (MAPKK), which in turn activates a particular MAPK. Environmental pressures activate JNK MAPKs and CSBP2 (p38α) MAPKs, whereas mitogenic stimulation activates ERK MAPKs. The MAPKKK, or MEKK4 protein, also known as MTK1, is encoded by this gene. At the C terminus of this protein lies a protein kinase catalytic domain. A regulatory domain can be present in the N-terminal nonkinase domain. In mammalian cells, MEKK4 expression triggered the JNK MAPK and CSBP2 (p38α) pathways, but not the ERK pathway. The customized MAP3K4 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

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The results have been consistent and reliable, providing crucial insights into MAP3K4 research. Mar 21 2020

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The MAP3K4 KD cell line allowed me to obtain accurate data. Aug 22 2023

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FAQ

Any questions about our products? Please visit our frequently asked questions page.

Published Data

Fig.1 MAP3K4 immunoprecipitations from MCF-7 breast cancer cells stimulated by HRG or sorbitol.

After lysates were exposed to either anti-MAP3K4 or anti-MAP3K4 antibody, Protein A-Sepharose was used to precipitate the mixture. Proteins that were immunoprecipitated were separated using SDS-PAGE. Using the designated antibodies, proteins were immunoblotted after being transferred to nitrocellulose membrane.

Ref: Garcia-Flores, Alejandro E., et al. "HER2/HER3 regulates lactate secretion and expression of lactate receptor mRNA through the MAP3K4 associated protein GIT1." Scientific Reports 9.1 (2019): 10823.

Pubmed: 31346208

DOI: 10.1038/s41598-019-46954-7

Research Highlights

The investigation identified several regulatory genes associated with NAFLD's hepatic lipid metabolism and showed that MAP3K4 was essential for NAFLD's hepatic lipogenesis.
He, Zhiyong, et al. "Identification of MAP3K4 as a novel regulation factor of hepatic lipid metabolism in non-alcoholic fatty liver disease." Journal of Translational Medicine 20.1 (2022): 1-13.
Pubmed: 36376950 DOI: 10.1186/s12967-022-03734-8

Bipotential gonads must commit to either an ovarian or a testicular fate in order to determine sex. In XY mice, gonadal sex reversal is caused by gene deletion of the kinase Map3k4, and the sex reversal phenotype is restored by transgenic re-expression of Map3k4.
Shendy, Noha AM, et al. "MAP3K4 kinase activity dependent control of mouse gonadal sex determination." Biology of Reproduction 105.2 (2021): 491-502.
Pubmed: 33912929 DOI: 10.1093/biolre/ioab083