mProX™ Human MAP3K2 Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-1122-KX1202	Magic™ Human MAP3K2 in Vitro Assay	Human		Kinase Assay

Product Information

Target Protein

MAP3K2

Target Family

Kinases/Enzyme Drug Discovery Assays and Products

Target Protein Species

Human

Host Cell Type

CHO-K1; HEK293

Target Classification

Kinase Cell Lines

Target Research Area

Cancer Research

Related Diseases

Lung Cancer. Among its related pathways are NGF Pathway and NF-KappaB Family Pathway

Gene ID

10746

UniProt ID

Q9Y2U5

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

Humans have an enzyme called mitogen-activated protein kinase kinase 2 (MEKK2/ERK kinase 2), which is encoded by the MAP3K2 gene. This gene codes for a protein that belongs to the family of serine/threonine protein kinases. The MAP kinase signaling pathway's other kinases are preferentially activated by this kinase. It has been demonstrated that this kinase directly phosphorylates and activates IkappaB kinases, contributing to the NF-kappa B signaling cascade. Additionally, it has been discovered that this kinase binds to and activates protein kinase C-related kinase 2, indicating that it may be involved in a controlled signaling pathway. The customized MAP3K2 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Gary

This MAP3K2 overexpression cell line is essential for our study. Mar 30 2020

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chat Patricia

I choose the MAP3K2 overexpression cell line with luminescent signals. Nov 10 2022

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FAQ

Any questions about our products? Please visit our frequently asked questions page.

Published Data

Fig.1 Map3k2 is required for maintaining the number of multiple intestinal epithelial cell subsets upon DSS-induced colitis.

Mice's colons stained with hematoxylin and eosin (H&E), on the left. Yes, the corresponding histology scores quantified. Arrows, penetrating the leukemia cells.

Ref: Wu, Ningbo, et al. "MAP3K2-regulated intestinal stromal cells define a distinct stem cell niche." Nature 592.7855 (2021): 606-610.

Pubmed: 33658717

DOI: 10.1038/s41586-021-03283-y

Research Highlights

These findings suggested that miR-186, at least in part, suppresses MAP3K2 expression to control the growth and spread of lung cancer. As a result, miR-186-MAP3K2 might offer a fresh and practical option for clinical treatment and NSCLC diagnosis.
Huang, Tonghai, et al. "MicroRNA-186 suppresses cell proliferation and metastasis through targeting MAP3K2 in non-small cell lung cancer." International journal of oncology 49.4 (2016): 1437-1444.
Pubmed: 27498924 DOI: 10.3892/ijo.2016.3637

There is no pharmacological management for acute lung injury (ALI), which results in a high fatality rate. Here, we discovered that pazopanib decreased edema in recipients of human lung transplants and ameliorated ALI symptoms as well as death in mouse models of ALI.
Yuan, Qianying, et al. "Pazopanib ameliorates acute lung injuries via inhibition of MAP3K2 and MAP3K3." Science translational medicine 13.591 (2021): eabc2499.
Pubmed: 33910977 DOI: 10.1126/scitranslmed.abc2499