mProX™ Human LTK Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-1122-KX1196	Magic™ Human LTK in Vitro Assay	Human		Kinase Assay

Product Information

Target Protein

LTK

Target Family

Kinases/Enzyme Drug Discovery Assays and Products

Target Protein Species

Human

Host Cell Type

Ba/F3; CHO-K1; HEK293

Target Classification

Kinase Cell Lines

Target Research Area

Autoimmune Research; Cancer Research

Related Diseases

Lupus Erythematosus and Her2-Receptor Positive Breast Cancer

Gene ID

4058

UniProt ID

P29376

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The human LTK gene encodes the enzyme leukocyte receptor tyrosine kinase. The ALK/LTK receptor family of receptor tyrosine kinases (RTKs), whose ligand is unknown, includes the protein that this gene encodes. closely associated with the RTK family of insulin receptors. Proteins phosphorylated at certain tyrosines play a crucial role in regulating a variety of processes that result in cell division and proliferation. For this gene, two alternatively spliced transcript variants encoding distinct isoforms have been identified. The customized LTK stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Cynthia

The LTK KD cell line enable us to develop new insights into its role in disease pathology. Sep 22 2022

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chat Matthew

We have found this human LTK cell line to be highly reliable and robust, producing consistent results. Jun 16 2022

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FAQ

Any questions about our products? Please visit our frequently asked questions page.

Published Data

Fig.1 LTK-F568L transforms BaF3 cells to cytokine-independence.

On Day 0, parental control cells (black circles) and BaF3 cells stably expressing LTK-F568L (red inverted triangles), LTK-R669Q (green triangles), or wildtype LTK (blue squares) were grown without IL-3. Using trypan blue exclusion, the total number of viable cells was calculated at each timepoint.

Ref: Roll, J. Devon, and Gary W. Reuther. "ALK-activating homologous mutations in LTK induce cellular transformation." PLoS One 7.2 (2012): e31733.

Pubmed: 22347506

DOI: 10.1371/journal.pone.0031733

Research Highlights

This is the first account of changes in LTK that have carcinogenic potential in malignancies. These findings suggest that lorlatinib may be used to treat NSCLC by targeting the CLIP1-LTK fusion.
Izumi, Hiroki, et al. "The CLIP1-LTK fusion is an oncogenic driver in non-small-cell lung cancer." Nature 600.7888 (2021): 319-323.
Pubmed: 34819663 DOI: 10.1038/s41586-021-04135-5

These results establish a physiological relationship between augmentor ligands and Ltk and show that specific augmentors can trigger iridophore development from neural crest-derived cells and pigment progenitor cells by activating Ltk in a tissue-specific setting.
Mo, Elizabeth S., et al. "Alk and Ltk ligands are essential for iridophore development in zebrafish mediated by the receptor tyrosine kinase Ltk." Proceedings of the National Academy of Sciences 114.45 (2017): 12027-12032.
Pubmed: 29078341 DOI: 10.1073/pnas.1710254114