mProX™ Human LRRC15 Stable Cell Line

Product Information

Target Family

Other Targets

Target Protein Species

Human

Host Cell Type

HEK293;CHO-K1;OVCAR-5;OVCAR-7

Target Classification

Other Targets Drug Discovery Assays and Products

Gene ID

Human:131578

UniProt ID

Human:Q8TF66

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

LRRC15, a transmembrane protein, has been studied in various contexts. In the context of SARS-CoV-2, LRRC15 has been found to interact with the receptor binding domain (RBD) of the spike protein, potentially modulating host cell receptiveness for the virus. In mesenchymal stem cells (MSCs), LRRC15 expression is correlated with high levels of stemness regulated by TWIST1, making it a useful marker for estimating stem cell quality in MSCs. In the context of colitis-associated colorectal cancer (CAC), LRRC15 is implicated in the inhibition of epithelial mesenchymal transition and induction of pyroptosis, suggesting its potential as a therapeutic target. Additionally, LRRC15 has been identified as a receptor for SARS-CoV-2 spike protein in fibroblasts, controlling antiviral and antifibrotic transcriptional programs. It is also a marker for a cluster of tumor-promoting cancer-associated fibroblasts (CAFs) called cancer-specific fibroblasts (CSFs), which may play a role in tumor progression.

Protocols

Please visit our protocols page.

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FAQ

chat Jordan Johnson (Verified Customer)

What is the significance of LRRC15 in cancer-associated fibroblasts? May 17 2023

chat Patrick Liam (Creative Biolabs Scientific Support)

LRRC15 is identified as a new marker of cancer-associated fibroblasts and cancers of mesenchymal origin, suggesting its potential as a target for antibody-drug conjugates in tumor stroma. May 17 2023

chat Cameron Jones (Verified Customer)

How does LRRC15 influence osteogenic differentiation? Aug 15 2023

chat Patrick Liam (Creative Biolabs Scientific Support)

LRRC15 plays a crucial role in osteogenesis of mesenchymal stem cells by modulating p65 cytoplasmic/nuclear translocation, indicating its importance in bone regeneration. Aug 15 2023

Published Data

Fig.1 Immunoblot analysis was conducted to assess LRRC15 expression in OVCAR5 NTC and sh1/sh2 KD cells, as well as in the control cells transfected with OVCAR7 EV, and cells overexpressing LRRC15 in Cl2 and Cl3.

KD LRRC15 expression clones were generated in OVCAR5 cells through the utilization of two distinct shRNAs (sh1 and sh2), while the NTC was employed as a control (left panel). Furthermore, LRRC15 overexpression clones were generated in the OVCAR7 cell line, which exhibited undetectable LRRC15 levels, resulting in the generation of OVCAR7 Cl2/Cl3 (LRRC15 overexpression) or EV (right panel).

Ref: Ray, Upasana, et al. "Targeting LRRC15 inhibits metastatic dissemination of ovarian cancer." Cancer research 82.6 (2022): 1038-1054.

Pubmed: 34654724

DOI: 10.1158/0008-5472.CAN-21-0622

Research Highlights

Mishra, Sarbani. et al. "Dissecting the Molecular Basis of Host Leucine-Rich Repeat Containing 15 Mediated Interaction with Receptor Binding Domain of SARS-CoV-2 Spike Protein: A Computational Approach." The journal of physical chemistry letters, 2023.
The role of leucine-rich repeat containing 15 (LRRC15) in the activity of SARS-CoV-2 pathways has been highlighted, indicating its potential involvement in the differential restriction of the virus. However, the structure and function of LRRC15 in relation to the receptor binding domain (RBD) of the SARS-CoV-2 spike protein and their interaction is not well understood. By utilizing advanced techniques such as AlphaFold2 and molecular dynamics simulations, it has been demonstrated that RBD can bind to different regions of LRRC15 with varying affinities. These findings suggest that both the receptor binding regions in RBD, particularly the receptor binding motif, play a critical role in determining the susceptibility of host cells to SARS-CoV-2 and their innate immune response. Further experimental studies are needed to fully understand the mechanisms and unique characteristics of this host receptor in the context of the COVID-19 pandemic, including its transmembrane and cytoplasmic domains.
Mishra, Sarbani. et al. "Dissecting the Molecular Basis of Host Leucine-Rich Repeat Containing 15 Mediated Interaction with Receptor Binding Domain of SARS-CoV-2 Spike Protein: A Computational Approach." The journal of physical chemistry letters, 2023.
Pubmed: 37781985 DOI: 10.1021/acs.jpclett.3c01443

Toriumi, Kensuke. et al. "LRRC15 expression indicates high level of stemness regulated by TWIST1 in mesenchymal stem cells." iScience, 2023.
In this study, the authors focused on investigating the role of the transcription factor TWIST1 in the stemness of mesenchymal stem cells (MSCs). They discovered a correlation between TWIST1 expression and the transmembrane protein LRRC15. Furthermore, they found that LRRC15-positive MSCs had higher expression levels of stemness-associated transcription factors and therapeutic cytokines. These cells also demonstrated improved therapeutic effects in a pulmonary fibrosis model. The study suggests that TWIST1 plays a critical role in the stemness of MSCs, and that LRRC15 could serve as a useful marker for assessing MSC quality prior to cell transplantation.
Toriumi, Kensuke. et al. "LRRC15 expression indicates high level of stemness regulated by TWIST1 in mesenchymal stem cells." iScience, 2023.
Pubmed: 37534184 DOI: 10.1016/j.isci.2023.106946