mProX™ Human ITK Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-1122-KX1175	Magic™ Human ITK in Vitro Assay	Human		Kinase Assay

Product Information

Target Protein

ITK

Target Family

Kinases/Enzyme Drug Discovery Assays and Products

Target Protein Species

Human

Host Cell Type

Jurkat; CHO-K1; HEK293

Target Classification

Kinase Cell Lines

Target Research Area

Cardiovascular Research; Reproductive Research; Autoimmune Research

Related Diseases

Lymphoproliferative Syndrome 1 and Agammaglobulinemia, X-Linked. Among its related pathways are Immune response NFAT in immune response and CD28 Signaling in T-Helper Cell

Gene ID

3702

UniProt ID

Q08881

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

Kinase tyrosine-protein Interleukin-2-inducible T-cell kinase, or ITK/TSK for short, is a protein that is encoded by the ITK gene in humans. ITK is a kinase that belongs to the TEC family and is highly expressed in T cells. An intracellular tyrosine kinase expressed in T-cells is encoded by this gene. It is believed that the protein contributes to T-cell differentiation and proliferation. For Th2 and Th17 cell growth and effector function, ITK is functionally significant. It was demonstrated that mice deficient in ITK were resistant to asthma. The customized ITK stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Nancy

The ITK KO cell line design is well-optimized, and the provided documentation is comprehensive. Apr 27 2021

chat Verified Customer

chat William

I would recommend this product to anyone who wants to research ITK. May 05 2020

chat Verified Customer

FAQ

Any questions about our products? Please visit our frequently asked questions page.

Published Data

Fig.1 ITK expression in four T-cell lines.

ITK and phosphorylated ITK (p-ITK) were expressed in Western lysates from Jurkat, MOLT4, CCRF CEM, and K299 both at baseline and after a 24-hour treatment with ITK inhibitors PF-06465469 (2 µM) and ibrutinib (2 µM).

Ref: Mamand, Sami, et al. "Comparison of interleukin-2-inducible kinase (ITK) inhibitors and potential for combination therapies for T-cell lymphoma." Scientific reports 8.1 (2018): 14216.

Pubmed: 30242208

DOI: 10.1038/s41598-018-32634-5

Research Highlights

T cells are the primary organs where ITK (IL-2-inducible tyrosine kinase), a member of the Tec family of kinases, is expressed. It is implicated in Th2, Th9, and Th17 responses, which regulate the synthesis of pro-inflammatory cytokines, as well as T cell growth and other TCR signaling events.
Lechner, Kristina S., Markus F. Neurath, and Benno Weigmann. "Role of the IL-2 inducible tyrosine kinase ITK and its inhibitors in disease pathogenesis." Journal of molecular medicine 98 (2020): 1385-1395.
Pubmed: 32808093 DOI: 10.1007/s00109-020-01958-z

Depending on the rater, segmentation time is shortened between 2.5 and 5 times compared to manual segmentation. Further tests in interactive 3D fetal ultrasound placenta segmentation demonstrate how the new technology can be used to a different issue domain.
Yushkevich, Paul A., et al. "User-guided segmentation of multi-modality medical imaging datasets with ITK-SNAP." Neuroinformatics 17 (2019): 83-102.
Pubmed: 29946897 DOI: 10.1007/s12021-018-9385-x