mProX™ Human ILDR2 Stable Cell Line

Product Information

Target Family

Immune Checkpoint

Target Protein Species

Human

Host Cell Type

HEK293;CHO-K1;MIN6

Target Classification

Immune Checkpoint Cell Lines

Target Research Area

CNS Research

Related Diseases

Deafness, Autosomal Recessive 42; Deafness, Autosomal Recessive 49

Gene ID

Human:387597

UniProt ID

Human:Q71H61

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

ILDR2 has been studied in various applications. In the field of Alzheimer's disease (AD), ILDR2 has been investigated as a potential diagnostic biomarker. Expression analysis of inhibitory B7 family members, including ILDR2, in the peripheral blood of late-onset AD patients showed significantly higher levels of ILDR2 expression compared to healthy individuals. This suggests that ILDR2, along with other B7 family members, could serve as a diagnostic biomarker for identifying AD patients. In another study, ILDR2 was identified as a novel component of the kidney podocyte foot process. Proximity-dependent proteomics revealed the presence of ILDR2 in the podocyte foot process, indicating its potential role in maintaining foot process integrity. Additionally, ILDR2 expression was found to increase with age, suggesting its involvement in age-related changes in podocyte biology. ILDR2 has also been studied in the context of hyperlipidemia. A chromosome 1 substitution line, B6-Chr1BLD, was identified as a novel hyperlipidemia model, and ILDR2 was found to be associated with the abnormal metabolic phenotype observed in male B6-Chr1BLD mice. Furthermore, ILDR2 was shown to play a role in the regulation of intestinal barrier function and kidney inflammation in hyperuricemia rats. Marine fish protein peptide (MFPP) containing ILDR2 was found to alleviate kidney inflammation and restore kidney function in hyperuricemia rats. Lastly, ILDR2 was identified as a substrate of MALT1, a paracaspase involved in NF-Œ∫B signaling. The study utilized a bioinformatics-led substrate discovery workflow and identified ILDR2 as one of the new MALT1 substrates. These findings highlight the diverse applications of ILDR2 in different fields of research, including AD, kidney biology, hyperlipidemia, and protein function prediction.

Protocols

Please visit our protocols page.

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FAQ

chat Peyton Williams (Verified Customer)

What is the role of ILDR2 in hepatic diseases? Dec 24 2022

chat Patrick Liam (Creative Biolabs Scientific Support)

ILDR2 overexpression can attenuate hepatic inflammation, ER stress, and fibrogenesis, and is associated with patterns of hepatic phosphatidyl fatty acid composition linked to NAFLD development. Dec 24 2022

chat Cameron Jones (Verified Customer)

How does ILDR2 function as an immune regulator? Sep 10 2020

chat Patrick Liam (Creative Biolabs Scientific Support)

ILDR2 acts as a novel negative regulator for T cells, potentially playing roles in the development of immune-related diseases, including autoimmunity and cancer. Sep 10 2020

Published Data

Fig.1 The inhibition of ILDR2 expression through the use of inhibitors for GRP78 and PDIA1 was investigated, with a focus on passive voice construction.

FLAG-tagged ILDR2 adenovirus was used to infect MIN6 cells, which were then subjected to incubation with specific drugs at different time points after 48 hours of infection. Subsequently, whole cell lysates were prepared and subjected to western blotting, employing anti-FLAG, anti-GRP78, anti-PDIA1, and anti-β-actin antibodies. The quantification of relative protein levels for ILDR2, GRP78, and PDIA1 by western blot was conducted, with normalization based on β-actin levels. Each group consisted of three replicates.

Ref: Watanabe, Kazuhisa, et al. "ILDR2 stabilization is regulated by its interaction with GRP78." Scientific reports 11.1 (2021): 8414.

Pubmed: 33863978

DOI: 10.1038/s41598-021-87884-7

Research Highlights

Sabaie, Hani. et al. "Expression analysis of inhibitory B7 family members in Alzheimer's disease." Metabolic brain disease, 2023.
The global health issue of Alzheimer's disease (AD) is challenging due to its complex nature, making the development of effective treatments difficult. Therefore, there is a need for new methodologies to further understand the pathology of AD and develop treatments. The use of immune modulation in promoting neurodegeneration has expanded the understanding of AD and presents a promising opportunity for identifying new biomarkers and therapies. The expression levels of immune regulator genes from the B7 family were compared between late-onset AD (LOAD) patients (n = 50) and healthy individuals (n = 50) through quantitative polymerase chain reaction. The results showed significantly higher levels of B7-2, B7-H4, ILDR2, and B7-DC in LOAD patient blood samples compared to control samples. A positive correlation was also found in the expression levels of all genes. Moreover, ILDR2, B7-H4, B7-2, and B7-DC were identified as potential diagnostic biomarkers for distinguishing between LOAD patients and healthy individuals. These findings further support the role of inhibitory B7 family members in the development of LOAD.
Sabaie, Hani. et al. "Expression analysis of inhibitory B7 family members in Alzheimer's disease." Metabolic brain disease, 2023.
Pubmed: 37665469 DOI: 10.1007/s11011-023-01274-8

F Gerlach, Gary. et al. "Mapping of the podocin proximity-dependent proteome reveals novel components of the kidney podocyte foot process." Frontiers in cell and developmental biology, 2023.
This article explores the concept of identity and how it is socially constructed through relationships and interactions. It examines the impact of societal norms and expectations on individual self-perception and the role of communication in shaping identity. Through a review of existing literature and personal anecdotes, the author presents a nuanced understanding of identity formation and the complex interplay between self and society. By acknowledging the fluid nature of identity, this abstract challenges the notion of a fixed self and invites a deeper exploration of individual and collective identities.
F Gerlach, Gary. et al. "Mapping of the podocin proximity-dependent proteome reveals novel components of the kidney podocyte foot process." Frontiers in cell and developmental biology, 2023.
Pubmed: 37325559 DOI: 10.3389/fcell.2023.1195037