mProX™ Human IL2RG Stable Cell Line

Product Information

Target Family

Immune Checkpoint

Target Protein Species

Human

Host Cell Type

HEK293;CHO-K1;Jurkat

Target Classification

Immune Checkpoint Cell Lines

Target Research Area

Immunology Research

Related Diseases

Severe Combined Immunodeficiency, X-Linked; Combined Immunodeficiency, X-Linked

Gene ID

Human:3561

UniProt ID

Human:P31785

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

IL2RG, also known as interleukin-2 receptor subunit gamma, has various applications in different fields of research. In the field of graft-versus-host disease (GVHD), IL2RG is found to be critical for sustaining T cell responses in target tissues. Its loss leads to aberrant expression of PD-1 in T cells, decreased numbers of tissue-infiltrating PD-1+ T cells, and impaired maintenance of progenitor-like T cells. However, PD-1 blockade can restore the capacity of IL2RG-ablated T cells to mediate GVHD. IL2RG is also required for protecting CD8+ T cells from PD-1 pathway-mediated suppression during GVHD. In the field of ovarian cancer, IL2RG is identified as one of the genes that participate in immune-related molecular mechanisms and regulate the expression of tumor-infiltrating immune cells. It is considered a potential immunotherapy target and prognostic marker for ovarian cancer. In the field of fish immunology, IL2RG is involved in the maturation of the immune system in medaka fish. The interactions between the host immune system and intestinal microorganisms in medaka depend on IL2RG, and reciprocal interactions between the microbiome and the intestinal tract are required for the maturation of the medaka immune system. In the field of periodontitis and alopecia areata, IL2RG is one of the candidate hub immuno-related genes (IRGs) that can be used for diagnosing the co-occurrence of these conditions. IL2RG, along with other hub genes, shows high diagnostic values for identifying patients with both periodontitis and alopecia areata. Finally, in the field of autoimmune rheumatic diseases, IL2RG is discussed in the context of sex bias in immune response. Females generally mount stronger immune responses and are more susceptible to autoimmune rheumatic diseases. IL2RG, located on the X-chromosome, is one of the genes critical for the immune response and may contribute to the sex differences in immune response and disease susceptibility. Overall, IL2RG has implications in GVHD, cancer immunotherapy, fish immunology, co-occurrence of diseases, and understanding sex differences in immune response.

Protocols

Please visit our protocols page.

Customer Reviews

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FAQ

chat Skyler Williams (Verified Customer)

What is the role of IL2RG in immune system function? Mar 24 2020

chat Patrick Liam (Creative Biolabs Scientific Support)

The IL2RG gene encodes the common gamma chain protein, essential for the function of several receptors in the immune system, playing a crucial role in immune responses. Mar 24 2020

chat Skyler Brown (Verified Customer)

How does IL2RG deficiency affect immune cell development? Nov 12 2020

chat Patrick Liam (Creative Biolabs Scientific Support)

IL2RG deficiency can lead to severe combined immunodeficiency (SCID) due to its critical role in the development and function of T cells, B cells, and natural killer cells. Nov 12 2020

Published Data

Fig.1 A significant increase in apoptosis of ITK-SYK+ Jurkat cells was observed when IL2RG knockdown was induced, in comparison to cells treated with si-control.

After 48 hours of transfection with siIL2RG or si-control, the TK-SYK+ Jurkat cells were subjected to Annexin V-PI staining for flow cytometry analysis, aiming to assess the proportion of apoptotic cells in both siIL2RG and si-control cell groups; the quantified results can be observed in the accompanying graph to the right.

Ref: Zhang, Lei‑Lei, et al. "Genome profiling revealed the activation of IL2RG/JAK3/STAT5 in peripheral T‑cell lymphoma expressing the ITK‑SYK fusion gene." International Journal of Oncology 55.5 (2019): 1077-1089.

Pubmed: 31545408

DOI: 10.3892/ijo.2019.4882

Research Highlights

Li, Qing. et al. "Correlation Analysis of Prognostic Gene Expression, Tumor Microenvironment, and Tumor-Infiltrating Immune Cells in Ovarian Cancer." Disease markers, 2023.
The study delves into the importance of tumor microenvironment (TME) research as a key factor in facilitating the development and advancement of effective biologic treatments for cancer. Focusing on ovarian cancer, the research explores the correlation between TME, the expression patterns of immune cells infiltrating the tumor, and prognostic gene expression. This analysis sheds light on the potential for TME-based approaches to enhance the efficacy of current cancer treatments and inform the development of novel therapies.
Li, Qing. et al. "Correlation Analysis of Prognostic Gene Expression, Tumor Microenvironment, and Tumor-Infiltrating Immune Cells in Ovarian Cancer." Disease markers, 2023.
Pubmed: 37841886 DOI: 10.1155/2023/9672158

Sakaguchi, Hiyori. et al. "Maturation of the medaka immune system depends on reciprocal interactions between the microbiota and the intestinal tract." Frontiers in immunology, 2023.
Previous research has focused on the relationship between host immune system and gut microorganisms in various animals, including fish. However, the specific impact on medaka, a commonly used fish model, remains unexplored. This study aims to analyze the effects of immunodeficiency on microbiota composition, as well as the role of gut bacteria in intestinal epithelial growth and immune function in medaka. Through a chronological examination of 8-month-old fish, findings suggest that immunodeficiency can significantly affect the microbiota and intestinal well-being.
Sakaguchi, Hiyori. et al. "Maturation of the medaka immune system depends on reciprocal interactions between the microbiota and the intestinal tract." Frontiers in immunology, 2023.
Pubmed: 37767090 DOI: 10.3389/fimmu.2023.1259519