mProX™ Human IL2RA Stable Cell Line

Product Information

Target Family

Immune Checkpoint

Target Protein Species

Human

Host Cell Type

HEK293;CHO-K1;UCSD-AML1;HL60

Target Classification

Immune Checkpoint Cell Lines

Target Research Area

Immunology Research

Related Diseases

Immunodeficiency 41 With Lymphoproliferation And Autoimmunity; Type 1 Diabetes Mellitus 10

Gene ID

Human:3559

UniProt ID

Human:P01589

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

IL2RA, also known as interleukin 2 receptor subunit alpha, has been studied in various medical contexts. In bipolar disorder (BD), IL2RA rs2104286 and IL2 rs2069762 polymorphisms have been found to be associated with the disorder and its clinical features. The study suggests that immuno-inflammatory pathways in the brain and environment contribute to the development of BD. In multiple myeloma (MM), IL2RA rs2104286, IL-2 rs2069762, and rs2069763 polymorphisms have been investigated for their effects on disease susceptibility, progression-free survival (PFS), and overall survival (OS). The study found that the IL2 rs2069762 TG genotype was associated with shorter PFS and OS, while the IL2 rs2069763 GG genotype was associated with shorter PFS. In kidney transplant recipients, the use of lymphocyte-depleting antibodies, such as anti-interleukin-2-receptor antibodies (IL2RA), has been examined in specific populations. The study found that compared to IL2RA, anti-thymocyte globulin (ATG) reduced the risk of acute rejection in standard and high-risk KTR, but did not decrease the risk of graft loss. The optimal induction therapy for kidney transplant recipients is still uncertain and requires further study. In oral cavity squamous cell carcinomas (OSCC), DNA methylation differences within INS, PTPN22, and IL2RA promoters have been observed in lymphocyte subsets. These differences in DNA methylation may contribute to an immunosuppressive tumor microenvironment in OSCC. The study also identified immune-associated long non-coding RNAs (lncRNAs) that may be involved in OSCC. In children with type 1 diabetes (T1D), the effect of T1D-susceptibility associated single nucleotide polymorphism (SNP) markers in INS, PTPN22, and IL2RA on CpG site methylation of their promoters in lymphocyte subsets was investigated. The study found associations between rs689 in INS and methylation at four CpG sites, as well as an association between rs12722495 in IL2RA and methylation at two CpG sites in B cells. These findings suggest that DNA methylation may mediate the genetic effects of these SNPs on T1D pathogenesis. Overall, IL2RA has been studied in the context of bipolar disorder, multiple myeloma, kidney transplantation, oral cavity squamous cell carcinomas, and type 1 diabetes, providing insights into its potential role in these conditions.

Protocols

Please visit our protocols page.

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FAQ

chat Jordan Brown (Verified Customer)

What is the function of IL2RA in immune regulation? Jan 03 2022

chat Patrick Liam (Creative Biolabs Scientific Support)

IL2RA (CD25) plays a critical role in immune regulation by recruiting inhibitory phosphatases to the cell membrane, thereby controlling STAT5 signaling in immune cells. Jan 03 2022

chat Peyton Williams (Verified Customer)

How does IL2RA expression impact cancer prognosis? Jul 28 2022

chat Patrick Liam (Creative Biolabs Scientific Support)

Elevated IL2RA expression in pancreatic ductal adenocarcinoma is associated with worse prognoses and increased immune infiltration, indicating its prognostic significance. Jul 28 2022

Published Data

Fig.1 The abundance and activity of regulators of proliferation, survival, cell-cycle activity, and apoptosis are influenced by IL2RA.

Immunoblot analyses were conducted to assess the abundance of key regulators involved in cell proliferation/survival, cell-cycle progression, and apoptosis in UCSD/AML1 and HL60 derivative cell lines. These experiments were carried out three times, yielding comparable results. Activating phosphorylations on Thr202 and Tyr204 were observed in ERK, while Ser473 exhibited activating phosphorylation in AKT, and Tyr694 in STAT5.

Ref: Nguyen, Chi Huu, et al. "IL2RA promotes aggressiveness and stem cell-related properties of acute myeloid Leukemia." Cancer research 80.20 (2020): 4527-4539.

Pubmed: 32873636

DOI: 10.1158/0008-5472.CAN-20-0531

Research Highlights

Pehlivan, Sacide. et al. "IL2RA rs2104286 and IL2 rs2069762 polymorphisms may be associated with bipolar disorder and its clinical findings." Nucleosides, nucleotides & nucleic acids, 2023.
The present study examined the potential involvement of immuno-inflammatory mechanisms in the development of bipolar disorder (BD). By analyzing the interleukin 2 (IL-2) gene polymorphisms, the researchers aimed to determine any potential correlation between these genetic variations and the risk of developing BD. The study included 200 BD patients and 200 healthy controls. The findings suggest that immuno-inflammatory pathways in the brain and environment may play a role in the etiology of this chronic affective disorder.
Pehlivan, Sacide. et al. "IL2RA rs2104286 and IL2 rs2069762 polymorphisms may be associated with bipolar disorder and its clinical findings." Nucleosides, nucleotides & nucleic acids, 2023.
Pubmed: 37843874 DOI: 10.1080/15257770.2023.2266820

Serin, Istemi. et al. "Effect of interleukin-2 (IL-2) polymorphisms on multiple myeloma: IL-2RA rs2104286, IL-2 rs2069762 and rs2069763 polymorphisms." Cytokine, 2023.
Interleukin-2 (IL-2) is a cytokine secreted by T helper type 1 cells and released after induction of T helper cells with major histocompatibility complexes or antigens presented by antigen presenting cells. Its activity and gene polymorphisms have been extensively studied in both solid and hematological malignancies. This study aimed to investigate the effects of IL-2RA rs2104286, IL-2 rs2069762 and rs2069763 polymorphisms on multiple myeloma (MM) susceptibility, progression-free survival (PFS) and overall survival (OS). A total of 300 MM patients and 170 healthy individuals were included, and the genotypes of the polymorphisms were compared to reveal their effects on disease susceptibility and survival. The results showed that patients with the TG genotype of IL-2 rs2069762 had a significantly shorter PFS and OS compared to others, while those with the GG genotype of IL-2 rs2069763 had a shorter PFS. These findings could have implications for IL-2 based therapies and MM epigenetics.
Serin, Istemi. et al. "Effect of interleukin-2 (IL-2) polymorphisms on multiple myeloma: IL-2RA rs2104286, IL-2 rs2069762 and rs2069763 polymorphisms." Cytokine, 2023.
Pubmed: 37832160 DOI: 10.1016/j.cyto.2023.156401