mProX™ Human IKBKE Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-1122-KX1170	Magic™ Human IKKε(IKBKE) in Vitro Assay	Human		Kinase Assay

Product Information

Target Protein

IKBKE

Target Family

Kinases/Enzyme Drug Discovery Assays and Products

Target Protein Species

Human

Host Cell Type

U87-MG; LN-229; CHO-K1; HEK293

Target Classification

Kinase Cell Lines

Target Research Area

Cancer Research; Infectious Research

Related Diseases

Viral Infectious Disease and Breast Cancer. Among its related pathways are 4-1BB Pathway and TNFR1 Pathway

Gene ID

9641

UniProt ID

Q14164

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

Nuclear factor inhibitor The IKBKE gene in humans codes for the enzyme kappa-B kinase subunit epsilon, sometimes referred to as I-kappa-B kinase epsilon or IKK-epsilon. It is a serine/threonine kinase that, via activating type I interferon, NF-kappa-B, and STAT signaling, regulates inflammatory responses to viral infection. implicated in the signaling of TNFA and inflammatory cytokines, such as interleukin-1. The potential therapeutic benefit of inhibiting IκB kinase (IKK) and IKK-related kinases, such as IKBKE (IKKε) and TANK-binding kinase 1 (TBK1), has been explored for the management of cancer and inflammatory disorders. The customized IKBKE stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Michael

The IKBKE cell line was delivered promptly, and it performed exceptionally well in my assays. Apr 22 2020

chat Verified Customer

chat Sarah

The IKBKE cell line is easy to handle and has shown consistent performance in various experimental setups. Aug 05 2023

chat Verified Customer

FAQ

Any questions about our products? Please visit our frequently asked questions page.

Published Data

Fig.1 Expression of IKBKE in cell lines and silence of IKBKE using shRNA in U87-MG and LN-229.

Real-time RT-PCR and western blotting were used to confirm the effects of IKBKE prohibition in U87-MG and LN-229 cells transfected with scrambled vector or IKBKE-shRNA lentivirus. These results demonstrated how strikingly obvious the effect of suppressing IKBKE was.

Ref: Lu, Jie, et al. "IKBKE regulates cell proliferation and epithelial-mesenchymal transition of human malignant glioma via the Hippo pathway." Oncotarget 8.30 (2017): 49502.

Pubmed: 28548934

DOI: 10.18632/oncotarget.17738

Research Highlights

These results demonstrate the functional significance of IKBKE in pancreatic cancer, provide credence to the assessment of IKBKE as a therapeutic target in PDAC, and propose IKBKE inhibition as a tactic to enhance the clinical efficacy of mTOR inhibitors.
Rajurkar, Mihir, et al. "IKBKE is required during KRAS-induced pancreatic tumorigenesis." Cancer research 77.2 (2017): 320-329.
Pubmed: 28069799 DOI: 10.1158/0008-5472.CAN-15-1684

All of these findings point to the possibility of using IKBKE as a target for metastatic breast cancer treatments and uncover a novel oncogenic role of IKBKE in controlling the abundance of snails, which in turn promotes breast cancer metastasis.
Xie, Wei, et al. "IKBKE phosphorylates and stabilizes Snail to promote breast cancer invasion and metastasis." Cell Death & Differentiation 29.8 (2022): 1528-1540.
Pubmed: 35066576 DOI: 10.1038/s41418-022-00940-1