mProX™ Human HIPK4 Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-1122-KX1165	Magic™ Human HIPK4 in Vitro Assay	Human		Kinase Assay

Product Information

Target Protein

HIPK4

Target Family

Kinases/Enzyme Drug Discovery Assays and Products

Target Protein Species

Human

Host Cell Type

NIH-3T3; CHO-K1; HEK293

Target Classification

Kinase Cell Lines

Target Research Area

Cancer Research

Related Diseases

Adenosquamous Lung Carcinoma

Gene ID

147746

UniProt ID

Q8NE63

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

Round spermatids elongate into chromatin-condensed spermatozoa during the amazing biological process known as mammalian spermiogenesis. In mice, spermiogenesis and male fertility depend on homeodomain-interacting protein kinase 4 (HIPK4); the signaling mechanisms that govern this process are poorly understood. HIPK4 is mainly expressed in round and early elongating spermatids, and Hipk4 knockout males are sterile, exhibiting characteristics consistent with oligoasthenoteratozoospermia. Hipk4 mutant sperm have reduced oocyte binding and are incompetent for in vitro fertilization, however they can still produce viable offspring via intracytoplasmic sperm injection. Defects in the filamentous actin (F-actin)-scaffolded acroplaxome during spermatid elongation and aberrant head morphologies in mature spermatozoa are shown by optical and electron microscopy of HIPK4-null male germ cells. The customized HIPK4 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat David

The mouse HIPK4 cell line was easy to use, and the customer service team was responsive and helpful. Apr 10 2020

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chat David

I am extremely satisfied with this HIPK4 KD cell line and the overall experience. Apr 15 2022

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FAQ

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Published Data

Fig.1 HIPK4 overexpression remodels the actin cytoskeleton in cultured cells.

Within two days of infection, fibroblasts that were neither transduced or that expressed the K40S mutant retained their spindle-like morphologies, while cells that expressed HIPK4 underwent a transformation into either a spherical or polygonal shape. The multinucleate polygonal HIPK4-overexpressing cells were probably caused by cytokinesis failure.

Ref: Crapster, J. Aaron, et al. "HIPK4 is essential for murine spermiogenesis." elife 9 (2020): e50209.

Pubmed: 32163033

DOI: 10.7554/eLife.50209

Research Highlights

Manchette protein RIMS-binding protein 3 is phosphorylated under the control of HIPK4, which is also involved in forming the sperm head and male fertility.
Liu, Xiaofei, et al. "Homeodomain-interacting protein kinase HIPK4 regulates phosphorylation of manchette protein RIMBP3 during spermiogenesis." Journal of Biological Chemistry 298.9 (2022).
Pubmed: 35931115 DOI: 10.1016/j.jbc.2022.102327

Hipk4 mutant sperm can nonetheless create healthy offspring by intracytoplasmic sperm injection, despite having decreased oocyte binding and being incompetent for in vitro fertilization. Defects in the filamentous actin (F-actin)-scaffolded acroplaxome during spermatid elongation and aberrant head morphologies in mature spermatozoa are shown by optical and electron microscopy of HIPK4-null male germ cells.
Crapster, J. Aaron, et al. "HIPK4 is essential for murine spermiogenesis." elife 9 (2020): e50209.
Pubmed: 32163033 DOI: 10.7554/eLife.50209