mProX™ Human HIPK3 Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-1122-KX1164	Magic™ Human HIPK3 in Vitro Assay	Human		Kinase Assay

Product Information

Target Protein

HIPK3

Target Family

Kinases/Enzyme Drug Discovery Assays and Products

Target Protein Species

Human

Host Cell Type

HCT116; SW480; CHO-K1; HEK293

Target Classification

Kinase Cell Lines

Target Research Area

CNS Research; Reproductive Research

Related Diseases

Huntington Disease

Gene ID

10114

UniProt ID

Q9H422

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The human HIPK3 gene encodes the enzyme homodomain-interacting protein kinase 3. According to descriptions, HIPK3 is a serine/threonine kinase that belongs to a family of nuclear kinases that co-repress homeodomain transcription factors. HIPK3 can counteract Myc/Ras-induced transformation and stimulate MDM2's transcriptional regulation mediated by p53. It's possible that HIPK3 is crucial for controlling the p53's apoptotic activity. Tumorigenesis may be promoted and apoptosis may be inhibited by HIPK3 inhibition. The customized HIPK3 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Nancy

The HIPK3 cell line is easy to use, and the provided protocols are clear and straightforward. Aug 22 2023

chat Verified Customer

chat Melissa

The HIPK3 overexpression cell line is approximately lower than other brands of reagents and I got similar results. Apr 07 2020

chat Verified Customer

FAQ

Any questions about our products? Please visit our frequently asked questions page.

Published Data

Fig.1 HIPK3 is the target gene of 101-3p.

The HIPK3 protein level was significantly reduced by 101-3p in both HCT116 and SW480. Nevertheless, in these two cell lines, 101-3p mimic had no effect on the amount of circHIPK3.

Ref: Tao, Lihuiping, et al. "HIPK3 inhibition by exosomal hsa-miR-101-3p is related to metabolic reprogramming in colorectal cancer." Frontiers in Oncology 11 (2022): 758336.

Pubmed: 35096570

DOI: 10.3389/fonc.2021.758336

Research Highlights

A growing body of research indicates that circular RNAs, or "circ RNAs," play critical roles in controlling the expression of genes. 6,154 unique circular RNAs were identified from human bladder cancer and normal bladder tissues using RNA-seq in this investigation.
Li, Yawei, et al. "Circ HIPK 3 sponges miR-558 to suppress heparanase expression in bladder cancer cells." EMBO reports 18.9 (2017): 1646-1659.

The results of this study verified the strong correlation between low HIPK3 expression in NSCLC tissues and low rates of survival following curative resection. HIPK3 has the potential to be a useful biomarker for predicting an NSCLC patient's survival.
Liu, Yifei, et al. "The expression level and prognostic value of HIPK3 among non-small-cell lung cancer patients in China." OncoTargets and therapy (2018): 7459-7469.
Pubmed: 30498360 DOI: 10.2147/OTT.S166878