mProX™ Human HIPK2 Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-1122-KX1163	Magic™ Human HIPK2 in Vitro Assay	Human		Kinase Assay

Product Information

Target Protein

HIPK2

Target Family

Kinases/Enzyme Drug Discovery Assays and Products

Target Protein Species

Human

Host Cell Type

HeLa; CHO-K1; HEK293

Target Classification

Kinase Cell Lines

Target Research Area

Cancer Research; Reproductive Research

Related Diseases

Keratoacanthoma and Encephalopathy, Neonatal Severe, Due To Mecp2 Mutations. Among its related pathways are Gene expression (Transcription) and Regulation of TP53 Activity

Gene ID

28996

UniProt ID

Q9H2X6

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The human HIPK2 gene encodes the enzyme homodomain-interacting protein kinase 2. As a serine/threonine protein kinase that interacts with homeodomain transcription factors, HIPK2 falls within this category. It is a member of the DYRK kinases, a family of protein kinases. HIPK2 is present in many different species and is controlled by multiple mechanisms in both apoptosis and gene expression. Through mutations in the speckle retention sequence, abnormal HIPK2 function has been linked to the pathophysiology of disorders such acute myeloid leukemia, myelodysplastic syndrome, and Alzheimer's disease due to HIPK2 hyperdegradation. Kidney fibrosis and cardiovascular disease have also been linked to loss of HIPK2 function, which is consistent with its tissue expression patterns. The customized HIPK2 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Linda

The performance of the human HIPK2 cell line was consistent. Feb 02 2023

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chat Rebecca

I have tested the HIPK2 KO cell line from Creative Biolabs as one of the most reliable and versatile options available. Mar 21 2020

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FAQ

Any questions about our products? Please visit our frequently asked questions page.

Published Data

Fig.1 MBR in HIPK2-depleted cells.

HeLa cells were transfected with siRNA specific to HIPK2 or a negative universal control. Four and five days after transfection, the cells were examined by WB using the specified Abs to confirm RNAi and by IF for MBR quantification using β-tubulin staining in conjunction with CEP55 as the MBR marker.

Ref: Sardina, Francesca, et al. "HIPK2 is required for midbody remnant removal through autophagy-mediated degradation." Frontiers in Cell and Developmental Biology 8 (2020): 572094.

Pubmed: 33043004

DOI: 10.3389/fcell.2020.572094

Research Highlights

The notion that HIPK2 is a major participant in many cancer forms is supported by all of these results, and as such, it may serve as a significant prognostic marker, a predictor of therapy response, or even a therapeutic target in and of itself.
Conte, Andrea, et al. "HIPK2 in cancer biology and therapy: Recent findings and future perspectives." Cellular Signalling 101 (2023): 110491.
Pubmed: 36241057 DOI: 10.1016/j.cellsig.2022.110491

By lowering P-P53, exercise-induced HIPK2 suppression prevents MI and lessens the death of cardiomyocytes. One potential innovative treatment strategy for MI is the inhibition of HIPK2.
Zhou, Qiulian, et al. "Exercise downregulates HIPK2 and HIPK2 inhibition protects against myocardial infarction." EBioMedicine 74 (2021).
Pubmed: 34837851 DOI: 10.1016/j.ebiom.2021.103713