mProX™ Human HASPIN Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-1122-KX1158	Magic™ Human Haspin(GSG2) in Vitro Assay	Human		Kinase Assay

Product Information

Target Protein

HASPIN

Target Family

Kinases/Enzyme Drug Discovery Assays and Products

Target Protein Species

Human

Host Cell Type

CHO-K1; HEK293

Target Classification

Kinase Cell Lines

Target Research Area

Cancer Research

Gene ID

83903

UniProt ID

Q8TF76

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

In eukaryotes, protein kinases are a diverse class of enzymes that are crucial to numerous cellular functions. A number of these proteins are active kinases; one such example is haspin, an unusual eukaryotic protein kinase that lacks sequence homology with other eukaryotic protein kinases and is exclusive to haploid germ cells. During mitosis, the serine/threonine kinase known as haspin binds to chromosomes and phosphorylates histone 3's threonine 3. Defective mitosis arises from overexpression or loss of haspin. Haspin inhibitors have been demonstrated to have strong anti-tumoral effects. Since threonine 3 of histone 3 is the only substrate for Haspin, inhibiting Haspin may not be as harmful as other anticancer drugs. The customized HASPIN stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Linda

Despite its competitive price, the HASPIN cell line exhibited excellent binding affinity and sensitivity comparable to more expensive alternatives. Feb 23 2021

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chat Shirley

The HASPIN cell line is a fantastic solution for researchers looking to save money without compromising quality. Aug 22 2020

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FAQ

Any questions about our products? Please visit our frequently asked questions page.

Published Data

Fig.1 HASPIN mRNA levels in breast cancer cell lines.

Given that HASPIN is an intronless gene, the PCR primer set was unable to discriminate between genomic and cDNA. Samples that tested negative for reverse transcriptase (RT) showed no amplification products, indicating that there was no genomic DNA contamination.

Ref: Nishida-Fukuda, Hisayo, et al. "Evaluation of the antiproliferative effects of the HASPIN inhibitor CHR-6494 in breast cancer cell lines." Plos one 16.4 (2021): e0249912.

Pubmed: 33852630

DOI: 10.1371/journal.pone.0249912

Research Highlights

Since threonine 3 of histone 3 is the only substrate for Haspin, inhibiting Haspin may not be as harmful as other anticancer drugs.
Amoussou, Nathalie Gisèle, et al. "Haspin: a promising target for the design of inhibitors as potent anticancer drugs." Drug Discovery Today 23.2 (2018): 409-415.
Pubmed: 29031622 DOI: 10.1016/j.drudis.2017.10.005

The foundation of cancer treatment is the selective elimination of cancerous cells without seriously harming healthy tissues, and new therapeutic targets for the management of tumors are continuously needed in current clinical practice. Most eukaryotes share the atypical protein kinase Haspin, which has been demonstrated to be especially active in cycling cells.
Quadri, Roberto, Sarah Sertic, and Marco Muzi-Falconi. "Roles and regulation of Haspin kinase and its impact on carcinogenesis." Cellular signalling 93 (2022): 110303.
Pubmed: 35278668 DOI: 10.1016/j.cellsig.2022.110303