mProX™ Human GSK3B Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-1122-KX1157	Magic™ Human GSK3β(GSK3B) in Vitro Assay	Human		Kinase Assay

Product Information

Target Protein

GSK3B

Target Family

Kinases/Enzyme Drug Discovery Assays and Products

Target Protein Species

Human

Host Cell Type

PANC-1; CHO-K1; HEK293

Target Classification

Kinase Cell Lines

Target Research Area

Cancer Research; Cardiovascular Research; Reproductive Research

Related Diseases

Familial Adenomatous Polyposis and Myocarditis. Among its related pathways are CTNNB1 S45 mutants aren't phosphorylated and IL-9 Signaling Pathways.

Gene ID

2932

UniProt ID

P49841

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The GSK3B gene in humans codes for the enzyme glycogen synthase kinase-3 beta (GSK-3 beta). The Gsk3b gene in mice encodes the enzyme. An elevated risk of bipolar disorder is linked to aberrant GSK-3 beta expression and control. Proline-directed serine-threonine kinase glycogen synthase kinase-3 (GSK-3) was first discovered to phosphorylate and inactivate glycogen synthase. Alpha (GSK3A) and beta are two isoforms that exhibit a high level of amino acid similarity. GSK3B has a role in the establishment of body patterns, neuronal cell development, and energy consumption. It could be a fresh target for ischemic stroke treatment. The customized GSK3B stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

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The GSK3B cell line's ease of use and reproducibility have significantly expedited our experiments. Jun 09 2021

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The consistent performance and reproducibility of GSK3B cell line have significantly boosted the quality of our data. Aug 09 2020

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FAQ

Any questions about our products? Please visit our frequently asked questions page.

Published Data

Fig.1 Expression, phosphorylation, and activity of GSK3b in the two cell lines.

GSK3b expression and the amounts of pGSK3b S9, pGSK3b Y216, and b-actin were measured by Western immunoblotting on protein extracts from HEK293 and PANC-1 cells. NRIKA identified GSK3b activity in PANC-1 and HEK293 cells.

Ref: Shimasaki, Takeo, et al. "Tu1882 Glycogen Synthase Kinase 3β Inhibition Sensitizes Pancreatic Cancer to Gemcitabine." Gastroenterology 5.144 (2013): S-871.

Pubmed: 22041920

DOI: 10.1007/s00535-011-0484-9

Research Highlights

Tau protein is phosphorylated by GSK3B, a key kinase that synthasizes glycogen synthase. One of the main characteristics of Alzheimer's disease (AD) is hyperphosphorylated tau. The exact function of GSK3B in AD pathogenesis remains unclear despite a great deal of research.
Kettunen, Petronella, et al. "Genetic variants of GSK3B are associated with biomarkers for Alzheimer's disease and cognitive function." Journal of Alzheimer's Disease 44.4 (2015): 1313-1322.
Pubmed: 25420549 DOI: 10.3233/JAD-142025

This work demonstrates a feasible route for further drug development endeavors and validates the viability of using oral medication to treat diabetes in order to restore β-cell mass.
Shen, Weijun, et al. "Inhibition of DYRK1A and GSK3B induces human β-cell proliferation." Nature communications 6.1 (2015): 8372.
Pubmed: 26496802 DOI: 10.1038/ncomms9372