mProX™ Human GSK3A Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-1122-KX1156	Magic™ Human GSK3α(GSK3A) in Vitro Assay	Human		Kinase Assay

Product Information

Target Protein

GSK3A

Target Family

Kinases/Enzyme Drug Discovery Assays and Products

Target Protein Species

Human

Host Cell Type

L6; CHO-K1; HEK293

Target Classification

Kinase Cell Lines

Target Research Area

Cancer Research

Related Diseases

Pancreatic Cancer and Hepatocellular Carcinoma. Among its related pathways are IL-9 Signaling Pathways and Activation of cAMP-Dependent PKA

Gene ID

2931

UniProt ID

P49840

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The GSK3A gene in humans codes for the enzyme glycogen synthase kinase-3 alpha. Similar to Drosophila'shaggy' (zeste-white3), glycogen synthase kinase 3-alpha is a multifunctional serine kinase that is involved in the regulation of multiple regulatory proteins, such as glycogen synthase and other transcription factors (e.g., JUN). Additionally, it participates in the signaling pathways for WNT and phosphoinositide 3-kinase, particularly PIK3CG. GSK3A function has been studied using model organisms. The customized GSK3A stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Jennifer

For GSK3A drug discovery, this is a good choice. Jun 23 2022

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chat Jessica

The cell line is ideal for helping to detect GSK3A. Oct 29 2021

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FAQ

Any questions about our products? Please visit our frequently asked questions page.

Published Data

Fig.1 GSK-3α/β expression in L6 cells expressing constitutively active GSK3S9A.

As a control, cells expressing the empty vector (EV) were employed. The expression of GSK3S9A was confirmed by immunoblotting GSK3β immunoprecipitates with a c-Myc antibody. This was followed by a reciprocal analysis to corroborate the results.

Ref: MacAulay, Katrina, et al. "Constitutive activation of GSK3 down-regulates glycogen synthase abundance and glycogen deposition in rat skeletal muscle cells." Journal of Biological Chemistry 280.10 (2005): 9509-9518.

Pubmed: 15632169

DOI: 10.1074/jbc.M411648200

Research Highlights

According to this research, GSK3A in sperm is necessary for male fertility. Furthermore, the glycine-rich N terminus of the GSK3A isoform in mammals suggests that this isoform has a unique role in maintaining proper sperm motility and fertility.
Bhattacharjee, Rahul, et al. "Targeted disruption of glycogen synthase kinase 3A (GSK3A) in mice affects sperm motility resulting in male infertility." Biology of reproduction 92.3 (2015): 65-1.
Pubmed: 25568307 DOI: 10.1095/biolreprod.114.124495

GSK-3, also known as Glycogen Synthase Kinase, is a promiscuous protein kinase that has a significant impact on various aspects of physiology and pathology, including as neurology, cellular metabolism, and lineage commitment, through the phosphorylation of its varied substrates.
Hermida, Miguel A., J. Dinesh Kumar, and Nick R. Leslie. "GSK3 and its interactions with the PI3K/AKT/mTOR signalling network." Advances in biological regulation 65 (2017): 5-15.
Pubmed: 28712664 DOI: 10.1016/j.jbior.2017.06.003