mProX™ Human GRK7 Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-1122-KX1155	Magic™ Human GPRK7(GRK7) in Vitro Assay	Human		Kinase Assay

Product Information

Target Protein

GRK7

Target Family

Kinases/Enzyme Drug Discovery Assays and Products

Target Protein Species

Human

Host Cell Type

CHO-K1; HEK293

Target Classification

Kinase Cell Lines

Target Research Area

Ocular Research

Related Diseases

Enhanced S-Cone Syndrome and Oguchi Disease 1. Among its related pathways are Activation of cAMP-Dependent PKA and Visual phototransduction

Gene ID

131890

UniProt ID

Q8WTQ7

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

Phototransduction is mediated by the serine/threonine-specific protein kinase known as G-protein-coupled receptor kinase 7 (GRK7). During high acuity color vision, this enzyme catalyzes the phosphorylation of cone (color) photopsins in retinal cones, mostly in the fovea. A member of the G protein-coupled receptor kinase family is GRK7. Mammalian retinal cone cells are the primary home of GRK7, where it phosphorylates light-activated photopsins, which are G protein-coupled receptors that detect red, green, and blue light. The capacity of the enzyme to identify color opsins in cone outer segment disk membranes is regulated by the post-translational modification of GRK7 through α-carboxyl methylation and geranylgeranylation. The customized GRK7 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

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The GRK7 cell line has proven valuable in various experimental setups, and its compatibility with different techniques makes it a versatile asset for our investigations. Sep 13 2022

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chat Michelle

I believe this is a simple, sensitive and effective GRK7 cell line. Mar 01 2021

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FAQ

Any questions about our products? Please visit our frequently asked questions page.

Published Data

Fig.1 Phosphorylation of bovine rhodopsin by GRK7 and GRK1.

Phosphorylating bovine ROS membranes included using extracts from either nontransfected (NT) HEK-293 cells or cells expressing ground squirrel GRK7 or bovine GRK1.

Ref: Weiss, Ellen R., et al. "The cloning of GRK7, a candidate cone opsin kinase, from cone-and rod-dominant mammalian retinas." Mol Vis 4.27 (1998): 865-885.

Pubmed: 9852166

Research Highlights

G protein-coupled receptor kinases (GRKs) phosphorylate photoactivated visual pigments in rods and cones of vertebrates, which is necessary for continued visual function.
Chrispell, Jared D., Yubin Xiong, and Ellen R. Weiss. "Grk7 but not Grk1 undergoes cAMP-dependent phosphorylation in zebrafish cone photoreceptors and mediates cone photoresponse recovery to elevated cAMP." Journal of Biological Chemistry 298.12 (2022).
Pubmed: 36273582 DOI: 10.1016/j.jbc.2022.102636

Zebrafish larvae that have either Grk1b or Grk7a removed have noticeably reduced recovery rates in response to subsequent light bursts. This decline was similar for grk1b- and grk7a-/-larvae and indicates, for the first time, that both Grk1b and Grk7a are important for the cessation of zebrafish cone visual signals.
Chrispell, Jared David, et al. "Contributions of Grk1b and Grk7a to the Cone Photoresponse in Zebrafish Larvae." Investigative Ophthalmology & Visual Science 57.12 (2016): 575-575.