mProX™ Human GRK3 Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-1122-KX1035	Magic™ Human BARK2(ADRBK2) in Vitro Assay	Human		Kinase Assay

Product Information

Target Protein

GRK3

Target Family

Kinases/Enzyme Drug Discovery Assays and Products

Target Protein Species

Human

Host Cell Type

CHO-K1; HEK293

Target Classification

Kinase Cell Lines

Target Research Area

Digestive and Renal Research; CNS Research

Related Diseases

Whim Syndrome 1 and Bipolar Disorder. Among its related pathways are Vesicle-mediated transport and GPCR downstream signalling

Gene ID

157

UniProt ID

P35626

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The human gene ADRBK2 codes for the enzyme G-protein-coupled receptor kinase 3 (GRK3). In order to facilitate the binding of an arrestin protein to the receptor, G protein-coupled receptor kinases phosphorylate active G protein-coupled receptors. By preventing receptor stimulation of heterotrimeric G protein transducer proteins, arrestin binding to phosphorylated, active receptors blocks their cellular signaling and causes receptor desensitization. Additionally, when an arrestin binds to a receptor, the receptor is guided into particular cellular internalization pathways, which eliminates the receptor from the cell surface and stops further activation. Receptor signaling via arrestin partner proteins is also made possible by arrestin binding to phosphorylated, active receptors. Thus, the GRK/arrestin system is the intricate signaling switch of G protein-coupled receptors. The customized GRK3 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Edison

The GRK3 cell line demonstrated remarkable specificity and sensitivity, which helped us unravel complex signaling pathways. Jun 18 2023

chat Verified Customer

chat Pandora

I recently utilized the GRK3 cell line from Creative Biolabs. This cell line proved to be an exceptional targeting tool for my research. Jan 07 2023

chat Verified Customer

FAQ

Any questions about our products? Please visit our frequently asked questions page.

Published Data

Fig.1 GRK3 was up-regulated in human metastatic prostate tumors and associated with elevated angiogenesis.

GRK3 IHC staining of prostatic cancerous and benign tissues. Stronger staining in advanced castration-resistant prostate cancer (C), comparable weak staining in localized prostate cancer (B), stronger staining in benign prostatic hyperplasia and negative luminal cells (A), stronger staining in skeletal metastasis around bone trabeculae (D), and the strongest staining observed in these tissue categories in soft tissue metastasis (E).

Ref: Li, Wenliang, et al. "GRK3 is essential for metastatic cells and promotes prostate tumor progression." Proceedings of the National Academy of Sciences 111.4 (2014): 1521-1526.

Pubmed: 24434559

DOI: 10.1073/pnas.1320638111

Research Highlights

By mediating CREB activation and acting as a new essential activator of NE phenotypes, these findings suggest that GRK3 is a novel factor that promotes NED in prostate cancer cells.
Sang, Meixiang, et al. "GRK3 is a direct target of CREB activation and regulates neuroendocrine differentiation of prostate cancer cells." Oncotarget 7.29 (2016): 45171.
Pubmed: 27191986 DOI: 10.18632/oncotarget.9359

In colon cancer, there was a specific overexpression of GRK3, which may mediate the proliferation of cancer cells, contribute to the disease's development, act as a poor prognostic sign, and could even be a novel target for treatment.
Jiang, Tao, et al. "Overexpression of GRK3, promoting tumor proliferation, is predictive of poor prognosis in colon cancer." Disease Markers 2017 (2017).
Pubmed: 29445249 DOI: 10.1155/2017/1202710