mProX™ Human GRIK2 Stable Cell Line

Nomura, Toshihiro. et al. "A pathogenic missense mutation in kainate receptors elevates dendritic excitability and synaptic integration through dysregulation of SK channels." The Journal of neuroscience : the official journal of the Society for Neuroscience, 2023.
A considerable number of rare variants that contribute to neurodevelopmental disorders (NDDs) are found in genes that are responsible for encoding synaptic proteins, particularly ionotropic glutamate receptors (iGluRs). Despite this, the ways in which these damaging missense variants impact brain function are often not well understood. The authors of this study sought to investigate the physiological effects of a specific missense mutation in an iGluR gene that has been linked to NDDs.
Nomura, Toshihiro. et al. "A pathogenic missense mutation in kainate receptors elevates dendritic excitability and synaptic integration through dysregulation of SK channels." The Journal of neuroscience : the official journal of the Society for Neuroscience, 2023.
Pubmed: 37802657   DOI: 10.1523/JNEUROSCI.1259-23.2023

Pan, Botao. et al. "Integrative multilevel exploration of the mechanism by which Er-Zhi-Wan alleviates the Parkinson's disease (PD)-like phenotype in the MPTP-induced PD mouse model." Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 2023.
The neuroprotective effects of Er-Zhi-Wan (EZW), a traditional Chinese formulation, in MPTP-induced Parkinson's disease (PD) models are not well understood. Thus, the aim of this study was to evaluate the neuroprotective effects of EZW in a mouse model of PD induced by MPTP. Using a combination of multilevel databases, network pharmacology, biological experiments, and LCMS/MS, the underlying pharmacological mechanisms of EZW in the prevention and treatment of PD were investigated. In vivo data demonstrated that pretreatment with EZW was able to protect against MPTP-induced motor dysfunction and rescue dopaminergic neurons from degeneration in mice. Network pharmacology analysis also revealed a complex, multi-component network that is responsible for the neuroprotective activity of EZW in PD treatment. Genes such as Grm2, Grm5, Drd2, and Grik2 were identified as important therapeutic targets, and subsequent experiments showed that EZW can regulate the mRNA levels of these genes and increase the phosphorylation of CREB to stimulate CREB signaling, thus reversing neuronal death after MPTP exposure. LC-MS/MS analysis further identified 53 compounds in EZW, highlighting the complexity of its neuroprotective network. This study provides important insight into the mechanism of EZW in MPTP-induced PD and supports its potential therapeutic role in neurodegenerative diseases.
Pan, Botao. et al. "Integrative multilevel exploration of the mechanism by which Er-Zhi-Wan alleviates the Parkinson's disease (PD)-like phenotype in the MPTP-induced PD mouse model." Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 2023.
Pubmed: 37348406   DOI: 10.1016/j.biopha.2023.115021