mProX™ Human GCK Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-1122-KX1153	Magic™ Human GCK in Vitro Assay	Human		Kinase Assay

Product Information

Target Protein

GCK

Target Family

Kinases/Enzyme Drug Discovery Assays and Products

Target Protein Species

Human

Host Cell Type

CHO-K1; HEK293

Target Classification

Kinase Cell Lines

Target Research Area

Diabetes Research; Reproductive Research

Related Diseases

Hyperinsulinemic Hypoglycemia, Familial, 3 and Maturity-Onset Diabetes Of The Young, Type 2. Among its related pathways are glycolysis (BioCyc) and trehalose degradation

Gene ID

2645

UniProt ID

P35557

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The GCK gene codes for the production of the glucokinase protein. This protein is crucial to the body's process of breaking down carbohydrates. The liver and the pancreatic beta cells contain the majority of the enzyme glucosekinase. The hormone insulin, which is produced and released by beta cells, helps control blood glucose levels by limiting the amount of glucose that enters cells from the bloodstream and is used as fuel. As a sensor, glucose kinase detects an increase in blood glucose levels and aids in triggering the release of insulin from beta cells to regulate them. Glucokinase in the liver assists in determining when more glucose should be ingested and transformed into glycogen, a significant source of energy that is stored in the body. The customized GCK stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Ashley

This is indeed a powerful GCK cell line. Jan 08 2020

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chat Jason

I can use the GCK cell line to direct measurement when testing complex biological samples. Jun 29 2020

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FAQ

Any questions about our products? Please visit our frequently asked questions page.

Published Data

Fig.1 V91L GCK expression in INS-1 832/13 cells induces D-glucose-dependent cell death.

SIN-SFFV-GCK or SIN-SFFV-GCK-V91L were transduced into HEK293T cells, and puromycin selection was performed after to confirm transgene expression. Using an immunoblot of transduced HEK293T cells, the expression of GCK and V91L GCK following vector transduction was verified.

Ref: Lu, Brian, et al. "Hyperinsulinemic hypoglycemia subtype glucokinase V91L mutant induces necrosis in β-cells via ATP depletion." Biochemistry and Biophysics Reports 17 (2019): 108-113.

Pubmed: 30623114

DOI: 10.1016/j.bbrep.2018.12.002

Research Highlights

This review covers various issues that are still unresolved in the areas of genetics, clinical diagnosis, and therapy of this particular kind of monogenic diabetes. It also provides updated information on GCK-MODY.
Hulín, Jakub, et al. "Clinical implications of the glucokinase impaired function-Gck-Mody today." Physiological Research 69.6 (2020): 995.
Pubmed: 33129248 DOI: 10.33549/physiolres.934487

These results demonstrate that a high detection rate of GCK-MODY can be achieved based on clinical phenotype and that a significant proportion are mistreated with glucose-lowering treatments prior to genetic testing.
Carmody, David, et al. "GCK-MODY in the US National Monogenic Diabetes Registry: frequently misdiagnosed and unnecessarily treated." Acta diabetologica 53 (2016): 703-708.
Pubmed: 27106716 DOI: 10.1007/s00592-016-0859-8