mProX™ Human FRK Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-1122-KX1150	Magic™ Human FRK in Vitro Assay	Human		Kinase Assay

Product Information

Target Protein

FRK

Target Family

Kinases/Enzyme Drug Discovery Assays and Products

Target Protein Species

Human

Host Cell Type

MDA-MB-231; CHO-K1; HEK293

Target Classification

Kinase Cell Lines

Target Research Area

Cancer Research

Related Diseases

Co-Trimoxazole Allergy and Liver Benign Neoplasm. Among its related pathways are PIP3 activates AKT signaling and Innate Immune System

Gene ID

2444

UniProt ID

P42685

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

Encoded by the FRK gene in humans, fyn-related kinase (FRK, previously tyrosine protein kinase 5) is an enzyme. The TYR family of protein kinases includes the protein that this gene encodes. This nuclear protein tyrosine kinase may inhibit growth during the G1 and S phases of the cell cycle. Following its initial discovery in 1993, research on FRK suggested that the enzyme may have a tumor-suppressive role. Additional functional analysis of the tyrosine kinase in many cancer types implies that FRK might also be carcinogenic. In particular, knockdown or catalytic inhibition of FRK inhibits key cellular activities in pancreatic and brain malignancies, whereas ectopic expression of FRK decreases cell proliferation and migration in breast and liver cancer. The customized FRK stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Anthony

The affinity is very good and I definitely recommend this FRK cell line. May 27 2021

chat Verified Customer

chat Richard

We are satisfied with this FRK cell line and we will use it in our experimental projects. Jan 01 2020

chat Verified Customer

FAQ

Any questions about our products? Please visit our frequently asked questions page.

Published Data

Fig.1 Effect of FRK expression on cell proliferation and anchorage-independent growth.

MDA-MB-231 cells were retrovirally infected with vectors encoding either FRK Y497F (FRK-YF) or wild-type FRK (FRK-WT), from which monoclonal populations were generated and named accordingly. Through quantitative RT-PCR analysis, the expression of FRK mRNA in the designated stable cell lines was ascertained.

Ref: Ogunbolude, Yetunde, et al. "FRK inhibits breast cancer cell migration and invasion by suppressing epithelial-mesenchymal transition." Oncotarget 8.68 (2017): 113034.

Pubmed: 29348886

DOI: 10.18632/oncotarget.22958

Research Highlights

Fyn-related kinase (FRK), a non-receptor tyrosine kinase, belongs to the BRK family of kinases (BFKs) and shares a distant relationship with the Src family of kinases (SFKs).
Goel, Raghuveera Kumar, and Kiven Erique Lukong. "Understanding the cellular roles of Fyn-related kinase (FRK): implications in cancer biology." Cancer and Metastasis Reviews 35 (2016): 179-199.
Pubmed: 27067725 DOI: 10.1007/s10555-016-9623-3

By stimulating metabolic reprogramming, which ultimately encourages EMT and metastasis, FRK enhances the stemness of H1299 cells and hence plays an oncogenic function in lung cancer cells.
Zhang, Li, et al. "FRK plays an oncogenic role in non-small cell lung cancer by enhancing the stemness phenotype via induction of metabolic reprogramming." International Journal of Cancer 146.1 (2020): 208-222.
Pubmed: 31251822 DOI: 10.1002/ijc.32530