mProX™ Human ESRRB Stable Cell Line

Product Information

Target Protein

ESRRB

Target Family

Estrogen-related Receptor

Target Protein Species

Human

Host Cell Type

TS; CHO-K1; HEK293

Target Classification

Nuclear Receptor

Target Research Area

Auditory and Otology Research; CNS Research

Related Diseases

Deafness

Gene ID

2103

UniProt ID

O95718

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The ESRRB (Estrogen Related Receptor Beta) gene in humans encodes the nuclear receptor known as estrogen-related receptor beta (ERR-β), often referred to as ESRRB or NR3B2 (nuclear receptor subfamily 3, group B, member 2). In mammalian cells, it has been demonstrated that ESRRB is essential for the change from a naïve to a primed pluripotent state, and NANOG regulates ESRRB expression in this context. The customized ESRRB stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Rebecca

I recently purchased the human ESRRB overexpression cell line, and it has exceeded my expectations. Jun 18 2021

chat Verified Customer

chat Nancy

We are delighted with the versatility of ESRRB cell line as a research tool. Dec 25 2022

chat Verified Customer

FAQ

Any questions about our products? Please visit our frequently asked questions page.

Published Data

Fig.1 Effects of Fgf/Erk signalling inhibition on TS cell transcription factors.

Comparing cells treated with Mek inhibitor PD0325901 ('PD03') for 3, 12, and 24 hours to untreated controls, Western blot analysis revealed the lack of phosphorylated Erk1/2; levels of overall Erk1/2 stayed unaltered. After three hours of PD03 treatment, eSRRB was decreased, and after twelve hours, it was almost nonexistent.

Ref: Latos, Paulina A., et al. "Fgf and Esrrb integrate epigenetic and transcriptional networks that regulate self-renewal of trophoblast stem cells." Nature communications 6.1 (2015): 7776.

Pubmed: 26206133

DOI: 10.1038/ncomms8776

Research Highlights

These findings suggest that Esrrb might function as a bookmarking factor during mitosis, providing a different angle from which to view sequence-specific transcription factors' involvement in the epigenetic regulation of pluripotency, self-renewal, and genome reprogramming.
Festuccia, Nicola, et al. "Mitotic binding of Esrrb marks key regulatory regions of the pluripotency network." Nature cell biology 18.11 (2016): 1139-1148.
Pubmed: 27723719 DOI: 10.1038/ncb3418

Transcription factors (TFs) that cooperate in DNA binding incorporate the cellular environment to facilitate cell specification throughout the developmental process. The pluripotent identity of naive mouse embryonic stem cells can persist indefinitely, as they originate from the early stages of development.
Knudsen, Teresa E., et al. "A bipartite function of ESRRB can integrate signaling over time to balance self-renewal and differentiation." Cell Systems 14.9 (2023): 788-805.
Pubmed: 37633265 DOI: 10.1016/j.cels.2023.07.008