mProX™ Human EPHA7 Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-1122-KX1128	Magic™ Human EPHA7 in Vitro Assay	Human		Kinase Assay

Product Information

Target Protein

EPHA7

Target Family

Kinases/Enzyme Drug Discovery Assays and Products

Target Protein Species

Human

Host Cell Type

BJAB; CHO-K1; HEK293

Target Classification

Kinase Cell Lines

Target Research Area

Reproductive Research

Related Diseases

Brachydactyly-Syndactyly Syndrome and Syndactyly, Type V. Among its related pathways are GPCR Pathway and ERK Signaling

Gene ID

2045

UniProt ID

Q15375

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The human EPHA7 gene encodes the protein known as ephrin type-A receptor 7. This gene is a member of the protein-tyrosine kinase family's ephrin receptor subfamily. Developmental events have been linked to EPH and EPH-related receptors, especially in the nervous system. The extracellular region of receptors in the EPH subfamily usually has two repeats of fibronectin type III and a Cys-rich domain, in addition to a single kinase domain. Based on the similarity of their extracellular domain sequences and their propensity to bind ephrin-A and ephrin-B ligands, the ephrin receptors are classified into two classes. The customized EPHA7 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Elizabeth

The EPHA7 cell line is an excellent product that has exceeded my expectations. Jan 23 2022

chat Verified Customer

chat Robert

The EPHA7 cell line is easy to use, and the results obtained have been consistent and reliable. I would definitely recommend it to others. Nov 13 2020

chat Verified Customer

FAQ

Any questions about our products? Please visit our frequently asked questions page.

Published Data

Fig.1 EphA7 is the predominant Eph receptor for infection of BJAB cells by KSHV.

Using recombinant soluble KSHV or RRV 26-95 gH/gL complexes as bait, pulldown from BJAB and 293T lysate was achieved. The input levels were adjusted to match the wet cell pellet. Using the designated antibodies, precipitates were subjected to Western blotting analysis.

Ref: Groβkopf, Anna K., et al. "EphA7 functions as receptor on BJAB cells for cell-to-cell transmission of the Kaposi's sarcoma-associated herpesvirus and for cell-free infection by the related rhesus monkey rhadinovirus." Journal of virology 93.15 (2019): 10-1128.

Pubmed: 31118261

DOI: 10.1128/JVI.00064-19

Research Highlights

In human prostate tissues, there was a positive correlation between the phosphorylation of EphA7 and the expression of EphrinA5. In conclusion, by focusing on the PI3K/Akt signaling pathways, receptor phosphorylation of EphA7 at least partially suppresses the aggressiveness of PCa tumors.
Li, Shibao, et al. "Ligand-dependent EphA7 signaling inhibits prostate tumor growth and progression." Cell death & disease 8.10 (2017): e3122-e3122.
Pubmed: 29022918 DOI: 10.1038/cddis.2017.507

Across a variety of cancer types, EPHA7-MUT successfully predicted improved clinical outcomes in ICI-treated patients, suggesting that EPHA7-MUT may be used as an immune checkpoint inhibitor predictive biomarker.
Zhang, Zhen, et al. "EPHA7 mutation as a predictive biomarker for immune checkpoint inhibitors in multiple cancers." BMC medicine 19 (2021): 1-15.
Pubmed: 33526018 DOI: 10.1186/s12916-020-01899-x