mProX™ Human EPHA5 Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-1122-KX1126	Magic™ Human EPHA5 in Vitro Assay	Human		Kinase Assay

Product Information

Target Protein

EPHA5

Target Family

Kinases/Enzyme Drug Discovery Assays and Products

Target Protein Species

Human

Host Cell Type

KYSE150; KYSE450; CHO-K1; HEK293

Target Classification

Kinase Cell Lines

Target Research Area

Cancer Research; Ocular Research; Reproductive Research

Related Diseases

Lung Cancer Susceptibility 3 and Leber Congenital Amaurosis 17. Among its related pathways are GPCR Pathway and ERK Signaling

Gene ID

2044

UniProt ID

P54756

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The EPHA5 gene in humans encodes the protein known as ephrin type-A receptor 5, or EPH receptor A5. This gene is a member of the protein-tyrosine kinase family's ephrin receptor subfamily. Developmental events have been linked to EPH and EPH-related receptors, especially in the nervous system. The extracellular region of receptors in the EPH subfamily usually has two repeats of fibronectin type III and a Cys-rich domain, in addition to a single kinase domain. Based on the similarity of their extracellular domain sequences and their propensity to bind ephrin-A and ephrin-B ligands, the ephrin receptors are classified into two classes. The customized EPHA5 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Jennifer

The EPHA5 cell line was an indispensable tool in my research. The cell line's stability and reproducibility were outstanding, allowing for reliable results across multiple experiments. Jul 18 2020

chat Verified Customer

chat Sarah

The EPHA5 cell line is of high quality and performs exceptionally well in my experiments. Jul 27 2021

chat Verified Customer

FAQ

Any questions about our products? Please visit our frequently asked questions page.

Published Data

Fig.1 Knockdown of EphA5 promoted the proliferation, migration, and invasion of ESCC cells in vitro.

In order to investigate the functions of EphA5, siRNA was transfected into KYSE150 and KYSE450 cells. The transfection markedly reduced the expression of EphA5 mRNA and protein.

Ref: Zhang, Rui, et al. "EphA5 knockdown enhances the invasion and migration ability of esophageal squamous cell carcinoma via epithelial-mesenchymal transition through activating Wnt/β-catenin pathway." Cancer cell international 20 (2020): 1-12.

Pubmed: 31956298

DOI: 10.1186/s12935-020-1101-x

Research Highlights

According to this study, EphA5 may be a target for epigenetic silencing in cases of primary prostate cancer. It may also be a useful prognostic indicator and therapeutic marker for the disease.
Li, Shibao, et al. "Downregulation of EphA5 by promoter methylation in human prostate cancer." BMC cancer 15 (2015): 1-10.
Pubmed: 25609195 DOI: 10.1186/s12885-015-1025-3

A novel monoclonal antibody targeting human EphA5 enhanced the radiosensitivity of lung cancer cells and human lung cancer xenografts, resulting in a notable extension of survival, indicating the possibility of translational uses.
Staquicini, Fernanda I., et al. "Receptor tyrosine kinase EphA5 is a functional molecular target in human lung cancer." Journal of Biological Chemistry 290.12 (2015): 7345-7359.
Pubmed: 25623065 DOI: 10.1074/jbc.M114.630525