mProX™ Human EPHA4 Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-1122-KX1125	Magic™ Human EPHA4 in Vitro Assay	Human		Kinase Assay

Product Information

Target Protein

EPHA4

Target Family

Kinases/Enzyme Drug Discovery Assays and Products

Target Protein Species

Human

Host Cell Type

SCP2; MDA-MB-231; CHO-K1; HEK293

Target Classification

Kinase Cell Lines

Target Research Area

CNS Research

Related Diseases

Amyotrophic Lateral Sclerosis 1 and Duane Retraction Syndrome. Among its related pathways are GPCR Pathway and ERK Signaling

Gene ID

2043

UniProt ID

P54764

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The EPHA4 gene in humans encodes the protein known as ephrin type-A receptor 4, or EPH receptor A4. This gene is a member of the protein-tyrosine kinase family's ephrin receptor subfamily. Developmental events have been linked to EPH and EPH-related receptors, especially in the nervous system. The extracellular region of receptors in the EPH subfamily usually has two repeats of fibronectin type III and a Cys-rich domain, in addition to a single kinase domain. Based on the similarity of their extracellular domain sequences and their propensity to bind ephrin-A and ephrin-B ligands, the ephrin receptors are classified into two classes. The customized EPHA4 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Michelle

This is a very reliable EPHA4 cell line with high sensitivity and specificity, suitable for experiments such as immunofluorescence microscopy and flow cytometry. May 13 2021

chat Verified Customer

chat Gary

Recommended for all labs based on my experience of using this EPHA4 cell line. Aug 16 2022

chat Verified Customer

FAQ

Any questions about our products? Please visit our frequently asked questions page.

Published Data

Fig.1 EPHA4 is required for TGFβ-mediated Migration in Human Breast Cancer Cell Lines.

In 6-well plates, confluence was reached after seeding stable MDA-MB-231 and SCP2 cells with either a scrambled or an EPHA4-specific shRNA infection. Using a wound-healing migration assay, migration was assessed after treatment with 0 or 5 ug/mL of TGFβ1 for a 24-hour period.

Ref: Hachim, Ibrahim Y., et al. "Transforming growth factor-beta regulation of ephrin type-A receptor 4 signaling in breast cancer cellular migration." Scientific reports 7.1 (2017): 14976.

Pubmed: 29101386

DOI: 10.1038/s41598-017-14549-9

Research Highlights

Future research in the field of neuro-regeneration pertaining to target-based medication discovery may draw from this study. Pathological and physiological processes linked to the interaction and elevation of ephrin and ephA4.
Verma, Meenal, Manjeet Chopra, and Hemant Kumar. "Unraveling the Potential of EphA4: A Breakthrough Target and Beacon of Hope for Neurological Diseases." Cellular and Molecular Neurobiology 43.7 (2023): 3375-3391.
Pubmed: 37477786 DOI: 10.1007/s10571-023-01390-0

A collection of neurodegenerative disorders that are deadly and have no known treatment is known as motor neuron disease (MND). Since one of the clinical features of MND is progressive motor neuron cell death, substances that shield these cells make appealing therapeutic targets.
Zhao, Jing, et al. "Role of EphA4 in mediating motor neuron death in MND." International Journal of Molecular Sciences 22.17 (2021): 9430.
Pubmed: 34502339 DOI: 10.3390/ijms22179430