mProX™ Human EPHA3 Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-1122-KX1124	Magic™ Human EPHA3 in Vitro Assay	Human		Kinase Assay

Product Information

Target Protein

EPHA3

Target Family

Kinases/Enzyme Drug Discovery Assays and Products

Target Protein Species

Human

Host Cell Type

DLD1; CHO-K1; HEK293

Target Classification

Kinase Cell Lines

Target Research Area

Infectious Research

Related Diseases

Wet Beriberi and Thiamine Deficiency Disease. Among its related pathways are GPCR Pathway and ERK Signaling

Gene ID

2042

UniProt ID

P29320

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The EPHA3 gene in humans encodes the protein known as EPH receptor A3, or ephrin type-A receptor 3. This gene is a member of the protein-tyrosine kinase family's ephrin receptor subfamily. Developmental events have been linked to EPH and EPH-related receptors, especially in the nervous system. The extracellular region of receptors in the EPH subfamily usually has two repeats of fibronectin type III and a Cys-rich domain, in addition to a single kinase domain. Based on the similarity of their extracellular domain sequences and their propensity to bind ephrin-A and ephrin-B ligands, the ephrin receptors are classified into two classes. A protein that binds ephrin-A ligands is encoded by this gene. For this gene, two alternatively spliced transcript variants have been identified. The customized EPHA3 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

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This EPHA3 cell line has saved us time and resources. Our team is excited to delve deeper into our investigations with this versatile tool. Jun 24 2023

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I think this EPHA3 cell line is better than many similar products already on the market. Jan 17 2022

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FAQ

Any questions about our products? Please visit our frequently asked questions page.

Published Data

Fig.1 Inducible EPHA3 overexpression in colon cancer cell lines.

Western blot shows LS174T and DLD1 cells that were transfected with pLenti/TO-EPHA3 or the control empty vector (EV) and treated with the appropriate doxycycline doses for 48 hours, demonstrating the amounts of EPHA3 protein in the cells.

Ref: Andretta, Elena, et al. "Investigation of the role of tyrosine kinase receptor EPHA3 in colorectal cancer." Scientific reports 7.1 (2017): 41576.

Pubmed: 28169277

DOI: 10.1038/srep41576

Research Highlights

The transmembrane glycoprotein members of the tyrosine kinase receptor family are the erythropoietin-producing human hepatocellular (Eph) receptors. The phosphorylation of their tyrosine kinase domain and the activation of the Eph receptor are the outcomes of the Ephs binding to different ephrin ligands.
London, Max, and Eugenio Gallo. "Critical role of EphA3 in cancer and current state of EphA3 drug therapeutics." Molecular Biology Reports 47 (2020): 5523-5533.
Pubmed: 32621117 DOI: 10.1007/s11033-020-05571-8

By binding and activating both human and mouse EphA3, the EphA3-specific monoclonal antibody (mAb), IIIA4, was able to identify the human EphA3 gene in a pre-B acute lymphoblastic leukemia (pre-B-ALL).
Charmsaz, Sara, et al. "EphA3 as a target for antibody immunotherapy in acute lymphoblastic leukemia." Leukemia 31.8 (2017): 1779-1787.
Pubmed: 27922598 DOI: 10.1038/leu.2016.371