mProX™ Human EEF2K Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-1122-KX1109	Magic™ Human EEF2K in Vitro Assay	Human		Kinase Assay

Product Information

Target Protein

EEF2K

Target Family

Kinases/Enzyme Drug Discovery Assays and Products

Target Protein Species

Human

Host Cell Type

HCC; CHO-K1; HEK293

Target Classification

Kinase Cell Lines

Target Research Area

Cancer Research

Related Diseases

Lung Cancer. Among its related pathways are MTOR signalling and Translation Insulin regulation of translation

Gene ID

29904

UniProt ID

O00418

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The human enzyme known as eukaryotic elongation factor-2 kinase, or eEF-2K, is encoded by the EEF2K gene. In the calmodulin-mediated signaling pathway, which connects several upstream signals to the control of protein synthesis, eEF-2 kinase is a highly conserved protein kinase. It inhibits the action of eukaryotic elongation factor 2 (EEF2) by phosphorylating it. This kinase's elevated activity is seen in many malignancies, making it a potential target for anti-cancer therapy. The expression of eEF-2K is frequently elevated in cancerous cells, such as those seen in the breast and pancreas, and it facilitates several processes such as cell division, survival, invasion, motility, and migration. Additionally, it has been proposed that eEF-2K, via controlling the production of neuronal proteins, may contribute to the quick antidepressant effects of ketamine. The customized EEF2K stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Karen

The EEF2K cell line has become a crucial tool in our lab, helping us gain deeper insights into angiogenesis and related processes. Mar 22 2023

chat Verified Customer

chat Amanda

As a researcher in vascular biology, I can confidently say that the EEF2K cell line is a fantastic tool. It has significantly improved the accuracy and precision of our experiments. Mar 08 2023

chat Verified Customer

FAQ

Any questions about our products? Please visit our frequently asked questions page.

Published Data

Fig.1 eEF2 kinase knock out in HCC cell lines leads to changes in proliferation.

eEF2K, peEF2, and eEF2 immunoblotting (with GAPDH as the loading control). There is no phosphorylated eEF2 seen in eEF2K -/-cells. Less kinase expression and thus less eEF2 phosphorylation are seen in eEF2K +/-cells.

Ref: Pott, Leona L., et al. "Eukaryotic elongation factor 2 is a prognostic marker and its kinase a potential therapeutic target in HCC." Oncotarget 8.7 (2017): 11950.

Pubmed: 28060762

DOI: 10.18632/oncotarget.14447

Research Highlights

According to these results, eEF2K may be a viable target for therapy and is a clinical signal of metastasis and prognostic importance for STAD survival.
Jiang, Mingxia, et al. "eEF2K as a novel metastatic and prognostic biomarker in gastric cancer patients." Pathology-Research and Practice 225 (2021): 153568.
Pubmed: 34352439 DOI: 10.1016/j.prp.2021.153568

The fact that pharmacological and genetic suppression of eEF2K can reverse the epileptic phenotype in a mouse model of human epilepsy suggests that eEF2K may be a new target for antiepileptic medications.
Heise, Christopher, et al. "eEF2K/eEF2 pathway controls the excitation/inhibition balance and susceptibility to epileptic seizures." Cerebral Cortex 27.3 (2017): 2226-2248.
Pubmed: 27005990 DOI: 10.1093/cercor/bhw075