mProX™ Human DYRK3 Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-1122-KX1107	Magic™ Human DYRK3 in Vitro Assay	Human		Kinase Assay

Product Information

Target Protein

DYRK3

Target Family

Kinases/Enzyme Drug Discovery Assays and Products

Target Protein Species

Human

Host Cell Type

CHO-K1; HEK293

Target Classification

Kinase Cell Lines

Target Research Area

CNS Research; Reproductive Research

Related Diseases

Neuroaspergillosis and Intellectual Developmental Disorder, Autosomal Dominant 7

Gene ID

8444

UniProt ID

O43781

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The enzyme known as dual specificity tyrosine-phosphorylation-regulated kinase 3 is encoded by the DYRK3 gene in humans. The DYRK family of dual-specificity protein kinases, which includes this gene product, catalyzes autophosphorylation on tyrosine and serine/threonine residues. Despite having comparable structural features, the members of this family have varied substrate specificities, which may indicate that they are involved in various cellular processes. It has been demonstrated that the encoded protein can catalyze the phosphorylation of histones H3 and H2B in vitro and autophosphorylate on tyrosine residues. Different isoforms are encoded by alternatively spliced transcript variants that have been discovered. The customized DYRK3 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Carol

The DYRK3 cell line was delivered promptly, and it performed exceptionally well in my assays. Jul 30 2021

chat Verified Customer

chat Charles

The DYRK3 cell line is easy to handle and has shown consistent performance in various experimental setups. Oct 26 2021

chat Verified Customer

FAQ

Any questions about our products? Please visit our frequently asked questions page.

Published Data

Fig.1 DYRK3 phosphorylates SNAPIN at the threonine 14 residue.

Following a 24-hour transfection of HEK293 cells with a plasmid encoding Myc-SNAPIN, three identical cell lysates were subjected to immunoprecipitation using an anti-Myc antibody. Through immunoblotting with an anti-Myc antibody, the correct expression of transiently produced SNAPIN in cell extracts was confirmed (Input).

Ref: Lee, Ye Hyung, et al. "DYRK3 phosphorylates SNAPIN to regulate axonal retrograde transport and neurotransmitter release." Cell Death Discovery 8.1 (2022): 503.

Pubmed: 36585413

DOI: 10.1038/s41420-022-01290-0

Research Highlights

By controlling the activity of a crucial enzyme involved in condensate disintegration and translation restoration, Hsp90 establishes a connection between stress adaption and cell development.
Mediani, Laura, et al. "Hsp90-mediated regulation of DYRK3 couples stress granule disassembly and growth via mTORC1 signaling." EMBO reports 22.5 (2021): e51740.
Pubmed: 33738926 DOI: 10.15252/embr.202051740

The prognosis for patients with glioblastoma multiforme (GBM), a malignant primary brain tumor, is not good. Even though surgery, chemotherapy, and radiation therapy are the usual treatments for gastric cancer of the breast (GBM), a tiny percentage of tumor cells that survive develop resistance to treatment and become more aggressive.
Kim, Kyeongmin, et al. "Dual specificity kinase DYRK3 promotes aggressiveness of glioblastoma by altering mitochondrial morphology and function." International Journal of Molecular Sciences 22.6 (2021): 2982.
Pubmed: 33804169 DOI: 10.3390/ijms22062982